两种类型CSTR生物制氢反应器的运行及产氢特性
Operation and Hydrogen Production Characteristics in Different Types of VCSTR and HCSTR
DOI: 10.12677/AEP.2018.83025, PDF,    科研立项经费支持
作者: 李峰哲*：城市水资源与水环境国家重点实验室(哈尔滨工业大学)，黑龙江 哈尔滨；金日成综合大学地球环境科学系，平壤；丁 杰, 赵兴丽, 任南琪：城市水资源与水环境国家重点实验室(哈尔滨工业大学)，黑龙江 哈尔滨；金正首, 金动律：金日成综合大学生命科学系，平壤
关键词: 厌氧；生物制氢；生物反应器；卧式CSTR反应器(HCSTR)；乙醇型发酵；Anaerobic； Biological Hydrogen Production； Bioreactors； Horizontal CSTR (HCSTR)； Ethanol-Type Fermentation
摘要: 为解决立式连续流搅拌槽式反应器(VCSTR)污泥易流失、低pH易崩溃的问题，设计并运行卧式连续流搅拌槽式反应器(HCSTR)，以红糖废水为基质进行混合微生物厌氧发酵制氢连续试验。污泥接种量为8.17 gVSS/L，温度为35℃ ± 1℃，HRT = 6 h，逐渐提高COD质量浓度以提高容积负荷(OLR)。在相同条件下运行立式连续流搅拌槽式反应器(HCSTR)作为对比，比较VCSTR与HCSTR乙醇型发酵产氢的运行效果，探求卧式反应器发酵产氢效能及优势。在最佳OLR (24 kg COD/(m3∙d))时，VCSTR与HCSTR系统的pH分别稳定在4.2和4.3左右；产气量分别达到86.1 L/d和132.8 L/d，其中氢气含量分别为47.30%和 51.24%；COD去除率维持在27.1%左右和24.3%左右。与VCSTR反应器相比, HCSTR反应器具有比较大有效容积和更高的单位时间底物处理能力，更适用于工业生产。
Abstract: The effect of reactor type on biogenic hydrogen production was investigated under the condition that the total volumes of two kinds of CSTR (Continuous Stirred Tank Reactor) were 32 L respec-tively. Experimental study on the anaerobic fermentation hydrogen production was carried out by using a vertical CSTR and a horizontal CSTR with a red basin as a substrate. The efficiency of the hydrogen production of VCSTR and HCSTR was compared, gradually increasing the organic load rate by increasing the COD concentration of influent (the inoculums was 8.17 gVSS/L, the temperature was 35˚C ± 1˚C, HRT = 6 h). The results show that the hydrogen production rates of the two reactors gradually increase with increasing OLR. The most suitable OLR for the two types of CSTR are 24 kg COD/(m3∙d), which the pH of VCSTR and HCSTR are maintained at about 4.2 and 4.3, respectively. Gas productions are 86.1 L/d and 132.8 L/d and hydrogen content 47.30% and 51.24% respectively. The COD removal rate of the VCSTR was maintained at 27.1%, and the COD removal rate of the HCSTR 24.3%. Compared to a VCSTR, the HCSTR is more suitable for industrial produc-tion because it has a larger effective volume and higher substrate processing capacity per unit time.
文章引用：李峰哲, 丁杰, 赵兴丽, 金正首, 金动律, 任南琪. 两种类型CSTR生物制氢反应器的运行及产氢特性[J]. 环境保护前沿, 2018, 8(3): 199-207. https://doi.org/10.12677/AEP.2018.83025

参考文献

[1] Lee, H.S., Vermaas, W.F.J. and Rittmann, B.E. (2010) Biological Hydrogen Production: Prospects and Challenges. Trends in Bio-technology, 28, 262-271. [Google Scholar] [CrossRef] [PubMed]
[2] Wong, Y.M., Wu, T.Y. and Juan, J.C. (2014) A Review of Sustainable Hydrogen Production Using Seed Sludge via Dark Fermentation. Renewable and Sustainable Energy Reviews, 34, 471-482. [Google Scholar] [CrossRef] 
[3] Li, Y.F., Zhang, J.W., Han, W., et al. (2011) Review on Engineering of Fer-mentative Biohydrogen Production. Advanced Materials Research, 183-185, 193-196.
[4] 王磊, 谢丽, 罗刚, 周琪. 反应器类型对生物厌氧发酵产氢的影响研究进展[J]. 中国农业科技导报, 2012, 14(2): 134-144.
[5] 才金玲, 王广策. 发酵生物制氢反应器研究进展[J]. 环境科学与技术, 2013, 36(6): 78-84.
[6] Li, C.L. and Fang, H.H.P. (2007) Fermentative Hydrogen Production from Wastewater and Solid Wastes by Mixed Cultures. Critical Reviews in Environmental Science and Technology, 37, 1-39. [Google Scholar] [CrossRef] 
[7] Lo, Y.C., Lee, K.S., Lin, P.J., et al. (2009) Bioreactors Configured with Dis-tributors and Carriers Enhance the Performance of Continuous Dark Hydrogen Fermentation. Bioresource Technology, 100, 4381-4387. [Google Scholar] [CrossRef] [PubMed]
[8] Lin, C.N., Wu, S.Y., Chang, J.S., et al. (2009) Biohydrogen Production in a Three-Phase Fluidized Bed Bioreactor Using Sewage Sludge Immobilized by Ethylene-Vinyl Acetate Copolymer. Bioresource Technology, 100, 3298-3301. [Google Scholar] [CrossRef] [PubMed]
[9] Zhang, Z.P., Adav, S.S., Show, K.Y., et al. (2008) Characteristics of Rapidly Formed Hydrogen-Producing Granules and Biofilms. Biotechnology and Bioengineering, 101, 926-936. [Google Scholar] [CrossRef] [PubMed]
[10] Zhang, Z.P., Show, K.Y., Tay, J.H., et al. (2008) Biohydrogen Production with Anaerobic Fluidized Bed Reactors—A Comparison of Biofilm-Based and Granule-Based Systems. International Journal of Hydrogen Energy, 33, 1559-1564. [Google Scholar] [CrossRef] 
[11] Hafez, H., Baghchehsaraee, B., Nakhla, G., et al. (2009) Comparative Assessment of Decoupling of Biomass and Hydraulic Retention Times in Hydrogen Production Bioreactors. International Journal of Hydrogen Energy, 34, 7603-7611. [Google Scholar] [CrossRef] 
[12] Younesi, H., Najafpour, G.K., Ku Ismail, S., et al. (2008) Biohydrogen Production in a Continuous Stirred Tank Bioreactor from Synthesis Gas by Anaerobic Photosynthetic Bacterium: Rhodopirillum rubrum. Bioresource Technology, 99, 2612-2619. [Google Scholar] [CrossRef] [PubMed]
[13] Ding, J., Wang, X., Zhou, X.F., et al. (2010) CFD Optimization of Conti-nuous Stirred-Tank (CSTR) Reactor for Biohydrogen Production. Bioresource Technology, 101, 7005-7013. [Google Scholar] [CrossRef] [PubMed]
[14] Ri, P.C., Ren, N.Q., Ding, J., Kim, J.S., et al. (2017) CFD Optimization of Horizontal Continuous Stirred-Tank (HCSTR) Reactor for Bio-Hydrogen Production. International Journal of Hydrogen Energy, 42, 9630-9640. [Google Scholar] [CrossRef] 
[15] 国家环保局《水与废水监测分析方法》编委会. 水与废水监测分析方法[S]. 北京: 中国环境科学出版社, 1997.
[16] 昌盛, 刘枫. 不同pH下糖蜜废水的厌氧产酸发酵类型及微生物群落结构解析[J]. 环境科学研究, 2016, 29(9): 1370-1377.
[17] 李建政, 任南琪, 秦智, 等. 产酸相反应器快速启动和乙醇型发酵菌群驯化[J]. 哈尔滨工业大学学报, 2002, 34(5): 591-594.
[18] Ren, N.Q., Wang, B. and Huang, J.C. (1997) Ethanol-Type Pefermentation from Carbohydrate in High Rate Acidogenic Reaetor. Biotechnology and Bioengineering, 54, 428-433. [Google Scholar] [CrossRef]