欧洲千里光叶片可培养内生细菌多样性分析
Diversity of Cultivable Endophytic Bacteria Isolated From Leaves of Senecio vulgaris L. (Asteraceae)
摘要: 菊科植物欧洲千里光在我国东北和西南地区是一种常见的外来入侵植物。为探究欧洲千里光可培养内生细菌的多样性，我们选择9株温室种植的健康的欧洲千里光植株为研究对象。通过叶片组织表面消毒处理，将内生菌通过涂布平板法和划线分离法，在牛肉膏蛋白胨培养基上进行分离、纯化，对纯化的菌株进行16s rDNA测序。我们一共得到了68株内生菌，并且鉴定出这些菌株属于3门3纲6科8属。其中，厚壁菌门的有芽孢杆菌属(Bacillus)、葡萄球菌属(Staphylococcus)；放线菌门的有微球菌属(Micrococcus)、节杆菌属(Arthrobacter)、细杆菌属(Microbacterium)；变形菌门的有假单胞菌属(Pseudomonas)、栖水菌属(Enhydrobacter)。从分离出的菌株数量来看，芽孢杆菌属(Bacillus)所占比例较大，高达63.2%；其次为节杆菌属(Arthrobacter)和假单胞菌属(Pseudomonas)，二者数量均占10.3%。本研究结果表明，欧洲千里光可培养内生细菌的多样性较为丰富，潜在的有益菌可能为欧洲千里光的入侵提供一定的帮助。
Abstract: Senecio vulgaris L. (Asteraceae) is an alien invasive plant species common in the northeast and southwest China. In order to explore the diversity of endophytic bacteria in S. vulgaris plants, nine healthy plants grown in greenhouse were used in the study. Isolates of endophytic bacteria were obtained from sterilized leaves of each individual plant. The phylogenetics analysis based on the 16s rDNA genes of the 68 endophytic bacteria isolates showed that the endophytic bacteria in the leaves of S. vulgaris plants could be classified as 7 genera belonging to Firmicutes, Actinobacteria and Proteobacteria. Naming They are: Bacillus, Staphylococcus (Firmicutes), Micrococcus, Arthrobacte, Microbacterium (Actinobacteria), Pseudomonas and Enhydrobacte (Proteobacteria). Among them, the Bacillus was the most dominant genera (66.7%), followed by Arthrobacter (10.6%) and Pseudomonas (7.6%). These results reveal that the leaves of S. vulgaris plants might harbor rather high diversity of endophytic bacteria and some of the endophytic bacteria were potential probiotics facilitating plant invasion.
文章引用：赵菁, 杨嘉麒, 程丹丹. 欧洲千里光叶片可培养内生细菌多样性分析[J]. 微生物前沿, 2018, 7(2): 58-64. https://doi.org/10.12677/AMB.2018.72007

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