槲皮素对水华鱼腥藻的生长抑制机理研究

Growth Inhibitory Mechanism of Quercetin on the Anabena flos-aquae

摘要: 本文选用黄酮类化合物槲皮素作为抑藻成分，将槲皮素与壳聚糖结合制作成槲皮素–壳聚糖抑藻剂小球。以水华鱼腥藻作为试验对象，研究不同剂量的槲皮素–壳聚糖抑藻剂小球对水华鱼腥藻的生长影响，并分析槲皮素抑藻的机理。研究表明，水华鱼腥藻藻液中加入槲皮素–壳聚糖抑藻剂小球培养96 h后，50 mg/L剂量组中的叶绿素a含量降低为0 h的83%；超氧化物歧化酶SOD活性比0h降低了51%；丙二醛MDA含量在36 h比0 h增加了54%。培养96 h后，三种叶绿素荧光参数Fv/Fm、Yield、ETRmax在50 mg/L剂量组，比0 h分别降低了83%、85%、78%。藻蓝蛋白和别藻蓝蛋白含量PC含量随着抑藻剂小球培养时间的增加，呈现递增趋势。槲皮素–壳聚糖抑藻剂小球会对水华鱼腥藻造成极大的氧化损伤，其抑制机理是通过抑制叶绿素a的合成，扰乱藻胆蛋白含量，破坏PS II反应中心，阻止光合作用的电子传递，使水华鱼腥藻光合作用效率降低，抑制了正常生长繁殖。

Abstract: In this paper, flavonoid quercetin was selected as the algal-inhibiting ingredient, and quercetin and chitosan were combined to form quercetin-chitosan algaecide beads. The effects of different doses of quercetin-chitosan algaecide beads on the growth of Anabena flos-aquae were studied. The mechanism of quercetin inhibition was analyzed. The study showed that the chlorophyll a content in the 50 mg/L dose group was reduced to 83% of 0 h after adding the quercetin-chitosan alginicide beads cultured in the algae for 96 h. The activity of superoxide dismutase was reduced by 51% compared with 0 h; the content of malondialdehyde increased by 54% at 36 h compared with 0 h. After 96 hours of culture, the three chlorophyll fluorescence parameters Fv/Fm, Yield, ETRmax in the 50 mg/L dose group were reduced by 83%, 85%, and 78%, respectively, compared to 0 h. The content of phycocyanin and allophycocyanin showed an increasing trend with the increase of algal culture time. The quercetin-chitosan algaecide pellets will cause great oxidative damage to Anabena flos-aquae. The inhibition mechanism is to inhibit the synthesis of chlorophyll a, disturb the phycobiliprotein content, and destroy the PS II reaction center. The electron transfer of photosynthesis reduces the photosynthetic efficiency of Anabena flos-aquae and inhibits normal growth and reproduction.