E3泛素–蛋白连接酶UPL3 DNA序列揭示德阳柿和油柿为栽培柿的最近缘物种
Diospyros oleifera and D. deyangensis Are Revealed as the Closest Relatives to D. kaki by E3 Ubiquitin-Protein Ligase UPL3 DNA Sequences
DOI: 10.12677/HJAS.2018.86100, PDF,  被引量    科研立项经费支持
作者: 李文清, 解孝满, 鲁仪增：山东省林木种质资源中心，山东 济南；杨 勇：西北农林科技大学园艺学院，陕西 杨凌；常 青：中国科学院新疆生态与地理研究所，新疆 乌鲁木齐；靳晓白：中国科学院北京植物园，北京；索志立*：中国科学院植物研究所系统与进化植物学国家重点实验室，北京
关键词: 柿属植物；E3泛素–蛋白连接酶；DNA序列标记；栽培品种；起源；遗传多样性；Diospyros； E3 Ubiquitin-Protein Ligase； DNA Marker； Cultivar； Origin； Genetic Diversity
摘要: 柿树的果实是著名传统食物。在过去几十年，由于城市化和环境破坏，柿树的种质资源受到威胁。柿科的柿属植物，在全世界约有400多种。为了保护和利用，急需评价柿属植物的遗传多样性。然而，由于形态和DNA标记数量少，灵敏度有限，柿属植物的鉴定仍然存在困难，开发分子鉴定方法仍然是全球的挑战。本文中，利用E3泛素–蛋白连接酶UPL3 DNA区域研发出新的DNA序列标记，可用于柿属植物分类。该DNA序列标记揭示，德阳柿和油柿是栽培柿的最近缘物种。对于深入理解栽培柿的起源、研究全球柿属植物的遗传多样性以及系统发育关系具有重要意义。
Abstract: Diospyros of the family Ebenaceae includes more than 400 species in the world. Fruits of persimmon plants are well-known traditional food. In the past decades, persimmon germplasm resources are threatened due to urbanization and environmental destruction. There is an urgent need to make an evaluation on the genetic diversity of Diospyros for conservation and utilization. However, due to the shortage and limited sensitivity of morphological and DNA markers, development of molecular identification tools for the Diospyros plants is still a global challenge. In this study, a new DNA marker from the E3 ubiquitin-protein ligase UPL3 gene region was found to be sensitive for discriminating Diospyros plants. Diospyros deyangensis and D. oleifera were revealed as the closest relatives to D. kaki by the E3 ubiquitin-protein ligase UPL3 DNA sequences. Our results would be of significant value for understanding the origin of cultivated persimmon cultivars and for future researches on global genetic diversity assessment and phylogeny of Diospyros plants.
文章引用：李文清, 杨勇, 解孝满, 鲁仪增, 常青, 靳晓白, 索志立. E3泛素–蛋白连接酶UPL3 DNA序列揭示德阳柿和油柿为栽培柿的最近缘物种[J]. 农业科学, 2018, 8(6): 657-673. https://doi.org/10.12677/HJAS.2018.86100

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