微生物产β-葡萄糖苷酶研究进展
Progress of β-Glucosidase from Microorganisms
DOI: 10.12677/AMB.2018.72010, PDF,  被引量    国家自然科学基金支持
作者: 常治帅, 兰 辉, 包亚莉, 刘占英*：内蒙古工业大学，内蒙古 呼和浩特
关键词: β-葡萄糖苷酶；基因克隆；酶活力测定；β-Glucosidase； Gene Cloning； Enzyme Activity Determination
摘要: β-葡萄糖苷酶能有效解除纤维二糖对纤维素酶活性的抑制，是限制纤维素彻底水解的重要因素。由于β-葡萄糖苷酶酶活相对较低、成本高等因素，通过基因工程手段对其定向改造，异源表达获得高酶活、耐热耐酸碱的β-葡萄糖苷酶，使其更适用于工业生产和应用。文中从β-葡萄糖苷酶的分类、微生物来源β-葡萄糖苷酶基因的克隆、酶活力测定方法等几方面对β-葡萄糖苷酶进行综述，以期为β-葡萄糖苷酶的研究提供借鉴。
Abstract: β-glucosidase can effectively decrease the inhibitory effect of cellobiose on cellulase activity, which is a bottleneck on the complete hydrolysis of cellulose. Because of its low activity and high cost, the β-glucosidase, which is highly resistant to acid and alkali, is more suitable for industrial production and application by means of genetic engineering technology and expressing in heterologous hosts. In this paper, there is a detailed summary about β-glucosidase in the classification and cloning about different sources of β-glucosidase gene, enzyme activity determination and so on, which provides theoretical support for enzyme researches.
文章引用：常治帅, 兰辉, 包亚莉, 刘占英. 微生物产β-葡萄糖苷酶研究进展[J]. 微生物前沿, 2018, 7(2): 79-86. https://doi.org/10.12677/AMB.2018.72010

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