不同分期血管瘤组织中端粒酶逆转录酶的表达及定位分析

Expression and Location Analysis of Human Telomerase Reverse Transcriptase in Infantile Hemangiomas at Different Stages

摘要: 目的：探讨端粒酶逆转录酶(hTERT)在不同时期婴幼儿血管瘤(His)中的表达和定位意义。方法：免疫组织化学(IHC)法检测hTERT在不同时期IHs组织及瘤周正常组织中的表达及定位，计算机采集图像，IHC半定量积分法及Image-Pro Plus(IPP) v6.0图像分析软件评估结果，相关统计方法分析数据。结果：hTERT在不同时期IHs组织中均有不同程度表达，呈散在分布；免疫组化阳性染色细胞主要是血管瘤内皮细胞，定位于胞浆及胞核；增殖期阳性细胞的平均光密度值明显高于消退期，具有显著性差异(P < 0.05)；不同时期IHs的阳性细胞平均光密度值与IHC半定量积分法最终得分之间呈显著正相关(r = 0.99, P = 0.00)。结论：增殖期IHs组织中存在较强的端粒酶活性。端粒酶活性来源定位主要在血管瘤内皮细胞的胞浆及胞核。

Abstract: PURPOSE: To investigate the expression and location of telomerase reverse transcriptase (hTERT) in infantile hemangiomas (IHs) at different stages. METHODS: Immunohistochemical method was used to detect the expression and location of hTERT in IHs tissues at different stages and normal tissues adjacenting to IHs respectively. Using computer collecting the images, the staining status was evaluated by IHC semi-quantitative integral method and Image-Pro Plus (IPP) V6.0 image analysis software; related statistical approaches were used to analyze the data. RESULTS: Different levels of hTERT expression were shown in all IHs samples with scattered staining pattern. The positive staining cells were mainly in hemangioma endothelial cells and localized in the cytoplasm and karyon. The average optical density in proliferating positive cells was significantly higher than that in involuting ones, and the difference was statistically significant (P < 0.05); the mean optical density of hTERT positive cells of IHs tissues at different stages was significantly correlated with the final score of IHC semi-quantitative integral method (r = 0.99, p = 0.00). CONCLUSIONS: Strongest telomerase activity exists in the proliferating stage of IHs. The position of telomerase activity basically locates in cytoplasm and karyon of hemangioma endothelial cells.