紫斑牡丹内生真菌分离培养分子鉴定及抑菌活性研究
Study on Identification and Antimicrobial Activity of Endophytic Fungi Separation from Paeonia rockii
DOI: 10.12677/WJF.2018.73011, PDF,    科研立项经费支持
作者: 田慧敏, 齐小剑, 尹元, 高原, 曾满意：赤峰学院生命科学学院，内蒙古 赤峰
关键词: 紫斑牡丹；内生菌；分子鉴定；抑菌实验；Paeonia rockii； Endophytic Fungi； Molecular Identification； Antimicrobial Activity
摘要: 本研究对紫斑牡丹的根、茎、叶内生真菌进行分离培养，根据形态学特征、培养特性及rDNA-ITS序列分析相结合的方法对分离到的菌种进行鉴定，并对其与黄瓜枯萎病原菌尖孢镰刀菌Fusarium oxysporum同时接种进行抑菌试验，以筛选抑制尖孢镰刀菌Fusarium oxysporum的内生菌菌株。结果表明，从紫斑牡丹中获得20株内生真菌，经形态和分子共鉴定出11种，J2为菊拟茎点霉菌Paraphoma chrysanthemicola (Hollós) Gruyter，J6HUI为Aspergillus calidoustus Varga，J7HEI为马铃薯刺盘孢Colletotrichum coccodes (Wallr.) S. Hughes，J8为木贼镰刀菌Fusarium equiseti (Corda) Sacc；G9无性型为多喙茎点霉Phoma multirostrata (P.N. Mathur, S.K. Menon & Thirum.) Dorenb. & Boerema，有性型为Boeremia sp.；G1、G6为番茄棘壳孢Pyrenochaeta lycopersici R.W. Schneid. & Gerlach，J1为Nodulosphaeria cirsii (P. Karst.) L. Holm，G5为Fusarium sp.，J6HUI2为Didymella glomerata (Corda) Qian Chen & L. Cai；J7HUI为Peyronellaea sp. (派伦霉属)，J1和J6HUI为中国新记录种，其中4株具有抑菌作用，G5、G9、J1和J2对黄瓜枯萎病具有不同程度的抑制作用，平均抑菌率分别为57%、57.2%、28.8%和54.8%。
Abstract: In this study, the endophytic fungi of root, stem, leaves from Paeonia rockii were isolated and cul-tured. According to the morphological and cultural characteristics and other biological characte-ristics, the isolates were identified by rDNA-ITS sequence analysis. The antibacterial test was per-formed on the isolates and Fusarium oxysporum by the plate-to-lime method to screen endophytic fungi with antibacterial activity. The results showed that 20 endophytic fungi were obtained from Paeonia rockii and 11 species were identified by rDNA-ITS sequence analysis. J2 was Paraphoma chrysanthemicola (Hollós) Gruyter (epigamous), Boeremia sp. (phorozoon); J6H was Aspergillus calidoustus Varga, and J7HEI was Colletotrichum coccodes (Wallr. S. Hughes); J8 is Gibberella intri-cans Wollenw (epigamous), Fusarium sp. (phorozoon); G9 is Phoma multirostrata (PN Mathur, SK Menon & Thirum.) Dorenb. & Boerema (phorozoon) Boeremia sp. (epigamous); G1 and G6 are Py-renochaeta lycopersici RW Schneid. & Gerlach, J1 is Nodulosphaeria cirsii (P. Karst.) L. Holm, G5 is Gibberella sp. (epigamous), and J6HUI2 is Didymella glomerata (Corda) Qian Chen & L. Cai; J7HUI is Peyronellaea sp. J1 and J6HUI are new to China, of which G5, G9, J1 and J2 can inhibit Fusarium oxysporum in different degrees, the average inhibition rates being 57%, 57.2%, 28.8% and 54.8%, respectively.
文章引用：田慧敏, 齐小剑, 尹元, 高原, 曾满意. 紫斑牡丹内生真菌分离培养分子鉴定及抑菌活性研究[J]. 林业世界, 2018, 7(3): 83-89. https://doi.org/10.12677/WJF.2018.73011

参考文献

[1] 郭良栋. 内生真菌研究进展[J]. 菌物系统, 2001, 20(1): 148-152.
[2] 官珊, 钟国华, 孙之潭, 等. 植物内生真菌的研究进展[J]. 仲恺农业技术学院学报, 2005, 18(1): 61-66.
[3] 李飞, 李春杰. 内生真菌对禾草类植物抗旱性的影响[J]. 草业科学, 2006, 23(3): 57-62.
[4] Funk, C.R., Halisky, P.M., Johnson, M.C., et al. (1983) Anendophytic Fungus and Resistance to od Webworms: Association in Lolium perenne L. Bio/technology, 1, 189-191.
[5] 郭绍霞, 张玉刚, 李敏, 等. 我国洛阳与菏泽牡丹主栽园区AM真菌多样性研究[J]. 生物多样性, 2007, 15(4): 425-431.
[6] White, J.F. and Cole, G.T. (1985) Endophyte Host Associations in Forage Grass III. In Vitro Inhibition of Fungi by Acremonium coenophialum. Mycologia, 77, 487-489. [Google Scholar] [CrossRef] 
[7] 兰琪, 姜广华, 吴文君. 农用植物内生真菌研究进展[J]. 世界农药, 2002, 24(3): 10-11.
[8] 李桂玲, 王建锋, 黄耀坚, 等. 几种药用植物内生真菌抗真菌活性的初步研究[J]. 微生物学通报, 2001, 28(6): 64-68.
[9] 杨润亚, 冯培勇, 李清. 植物内生真菌农药活性的研究进展[J]. 农药, 2006, 45(7): 440-444.
[10] 苗翠苹, 余莹, 陈有为, 等. 滇牡丹内生真菌的分离及其抑菌活性研究[J]. 中国药学杂志, 2011, 23(10): 738-741.
[11] 郑艳, 戴婧婧, 管玉鑫, 等. 凤丹内生真菌的分离及其抑菌活性研究[J]. 中国药学杂志, 2016, 41(1): 45-50.
[12] Huang, R., Jiang, B.G. and Li, X.N. (2018) Polyoxygenated Cyclohexenoids with Promising α-Glycosidase Inhibitory Activity Produced by Phomopsis sp. YE3250, an Endophytic Fungus Derived from Paeonia delavayi. Journal of Agricultural and Food Chemistry, 66, 1140-1146. [Google Scholar] [CrossRef] [PubMed]
[13] 张岚. 牡丹内生真菌多样性[D]: [硕士学位论文]. 洛阳: 河南科技大学, 2015: 1-62.
[14] 王皎. 滇牡丹内生真菌PR-35的鉴定及次生代谢产物研究[J]. 天然产物开发与研究, 2011, 37(11): 1339-1342.
[15] 赵丹. 牡丹根部茎部真菌病害及病原鉴定[D]: [硕士学位论文]. 洛阳: 河南科技大学, 2012: 1-78.

为你推荐