缝隙连接蛋白-43在膀胱出口部分梗阻的大鼠逼尿肌细胞膜中的表达

Connexin-43 Expressed on Cell Membranes in Rat Detrusor Muscle with Partial Bladder Outlet Obstruction Rats

摘要: 目的：探讨缝隙连接蛋白-43在膀胱出口部分梗阻大鼠逼尿肌细胞膜中表达的变化。方法：将33只雌性Wistar大鼠(10周龄)分为模型组和假手术组，两组大鼠分别于术后2、4和8周处死，同时进行膀胱内测压、缝隙连接数量、形态变化及缝隙连接蛋白-43的检测。结果：在模型组中缝隙连接的数量显著降低，并且术后4周缝隙连接蛋白-43在模型组大鼠逼尿肌细胞膜上的表达显著降低。模型组大鼠逼尿肌细胞膜上的缝隙连接数量也明显减少。结论：正常的排尿过程是通过逼尿肌细胞间的缝隙连接直接作用的结果。缝隙连接介导的细胞间通讯功能障碍可能是导致排尿功能障碍的原因。

Abstract: Objectives: To investigate alterations of gap junctional protein, connexin-43 (Cx-43), in the rat de-trusor muscle with partial bladder outlet obstruction (P-BOO). Methods: A total of 33 female Wistar rats (10 weeks old) were divided into a P-BOO group and a sham-operated control group and were killed at 2, 4, and 8 weeks after surgery. Cystometric investigation, the alteration of gap junction, and Cx-43 protein expression, which compose the gap junction, were examined. Results: The number of gap junctions was decreased in the P-BOO rat bladder. Furthermore, decreased cellular membrane expression of Cx-43 proteins was detected in rat detrusor muscle cells more than 4 weeks after surgery. The gap junctions of the detrusor muscle cell membranes were significantly fewer in number of the P-BOO rats with no detrusor contractions. Conclusions: These data suggest that the normal signals that contribute to voiding function could be transported directly through the gap junctions. Voiding dysfunction may be caused by the disruption of gap junctional intercel-lular communication.