七个玉米–大刍草重组自交系苗期抗旱性形态指标QTL定位
QTL Mapping of Drought Resistance in Seedling Stage of Seven Maize-Large Hum-ble Grass Recombinant Inbred Lines
DOI: 10.12677/HJAS.2018.88133, PDF,   
作者: 林景卫, 韩 笑, 周思雅, 李浩戈, 陈水森：沈阳农业大学生物技术学院，辽宁农业技术重点实验室，辽宁省植物基因工程技术研究中心，辽宁 沈阳；张立军, 崔震海, 阮燕晔*：沈阳农业大学生物技术学院，辽宁农业技术重点实验室，辽宁省植物基因工程技术研究中心，辽宁 沈阳;中国农业大学，国家玉米改良中心，北京市作物重点实验室，北京
关键词: 玉米；苗期抗旱性；QTL定位；重组自交系；Maize； Drought Resistance of Seedling Stage； QTL Mapping； Recombinant Inbred Lines
摘要: 为了实现玉米抗旱育种，探究各形态指标形状对玉米抗旱性的影响，本试验以玉米–大刍草重组自交系(MX RIL)群体为材料，对苗期调控玉米株高、根长、株鲜重、根鲜重、株干重、根干重以及根冠比7个性状的基因进行QTL定位分析。结果表明，株高、根长、根鲜重、株干重、根干重以及根冠比具有较高的遗传性，受基因型的影响。从定位到的11个QTL中，得到9个主效QTL，分别位于2，3，4，5和8号染色体上，其中1个来自株高性状，2个来自根长性状，2个来自根鲜重性状，1个来自株干重性状，2个来自根干重性状，1个来自根冠比性状；表型贡献率从6.0% (qMXRSR5)到10.25% (qMXDRW3-2)。本研究结果有助于进一步研究玉米尤其是苗期多种抗旱性状的遗传基础，并为通过分子育种提高玉米品种的品质提供理论依据和参考。
Abstract: In order to achieve drought resistance breeding of maize, the influence of various shape index shapes on maize drought resistance was explored. In this experiment, QTL mapping was carried out in the RIL group of maize-large straw recombinant inbred lines (MX) for the control of maize plant height, root length, plant fresh weight, root fresh weight, plant dry weight, root dry weight and root-crown ratio of 7 characters. The results showed that plant height, root length, root fresh weight, dry weight, root dry weight and root-crown ratio had higher heritability, which was influenced by genotype. Of the 11 QTLs to be located, 9 primary QTLs were obtained, they were located on 2nd, 3rd, 4th, 5th and 8th chromosome, of which 1 came from plant height traits, 2 from root length characters, 2 from root fresh weight traits, 1 from dry weight traits, 2 from root dry weight traits, and 1 from root and crown ratio traits. The phenotypic contribution rate is from 6.0% (qMXRSR5) to 10.25% (qmxdrw3-2). The results of this study are helpful to further study the genetic basis of various drought resistance traits in maize, especially in seedling stage, and provide theoretical basis and reference for improving the quality of maize varieties by molecular breeding.
文章引用：林景卫, 韩笑, 周思雅, 李浩戈, 陈水森, 张立军, 崔震海, 阮燕晔. 七个玉米–大刍草重组自交系苗期抗旱性形态指标QTL定位[J]. 农业科学, 2018, 8(8): 899-910. https://doi.org/10.12677/HJAS.2018.88133

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