CD4⁺T细胞Tim-3分子在急性期过敏性紫癜患儿外周血中的表达及其临床意义

The Expression and Clinical Significance of CD4⁺Tim-3⁺T Cell in Peripheral Blood for Acute Henoch Schonlein Purpura Patients

摘要: 目的：探讨Tim-3在急性期过敏性紫癜(Henoch Schonlein Purpura, HSP)患儿外周血CD4⁺细胞中的表达及其相关临床意义。方法：选取22名HSP急性期患儿及15名健康对照作为研究对象，流式细胞分析术分子检测各组研究对象外周血外周血CD4⁺Tim-3⁺T细胞比例。并分析该比例与相关临床表现的相关性。结果：HSP患儿急性期外周血CD4⁺ Tim-3⁺T细胞比例(3.03% ± 0.69%)下降较对照组(3.74% ± 0.62%)降低，二者比较结果有统计学意义(P < 0.05)。外周血CD4⁺Tim-3⁺T细胞比例与血沉水平呈负相关(r = −0.813, p < 0.05)，与皮疹持续天数(r = −0.063; P > 0.05)、肾损害(r = −0.208; P > 0.05)、ASO (r = −0.057; P > 0.05)、补体C3水平(r = −0.029; P > 0.05)及患儿受损系统的个数(r = 0.166; P > 0.05)无相关性。结论：HSP急性期患儿外周血CD4⁺Tim-3⁺T细胞比例降低。Tim-3通路有可能参与HSP发病，该指标也许可以作为衡量HSP疾病诊断及病情活动的参考指标。

Abstract: Objective: To identify the expression and clinical significance of Tim-3 in CD4⁺ T cells in HSP acute patients. Methods: 22 HSP acute patients and 15 health cases took part in this research. The fre-quencies of CD4⁺Tim-3⁺ T cells in peripheral blood were detected by Flow cytometry. It also ana-lyzed the relationship between CD4⁺Tim-3⁺ T cells and clinical features. Results: The frequencies of CD4⁺Tim-3⁺ T cells were lower in acute HSP patients (3.03% ± 0.69%) than in healthy controls (3.74 ± 0.62%). The frequencies of CD4⁺Tim-3⁺ T cells were negative related with ESR in HSP acute patients (r = −0.813, p < 0.05), but had no correlation with rash duration in days (r = −0.063; P > 0.05), kidney lesions (r = −0.208; P > 0.05), ASO (r = −0.057; P > 0.05), C3 (r = −0.029; P > 0.05) and the numbers with lesion systems (r = 0.166; P > 0.05). Conclusion: The frequencies of CD4⁺Tim-3⁺ T cells in acute HSP acute patients reduced. The passage of Tim-3 maybe involved the onset of HSP.