解淀粉芽孢杆菌的培养基优化和放大研究

Research on the Optimization of Fermentation Medium and Scale-Up Technology of Bacillus amyloliquefaciens

摘要: 采用PB设计和正交试验设计优化解淀粉芽孢杆菌发酵培养基，在50L发酵罐发酵条件基础上采用以体积溶氧系数为基准的放大法进行了比拟放大。结果表明，(NH₄)₂SO₄ 、玉米粉、玉米浆和葡萄糖的质量浓度是影响有效活菌数的主要因素，最优组合为玉米粉8 g/L、葡萄糖12 g/L、(NH₄)₂SO₄ 0.8 g/L、玉米浆7 g/L；50 L发酵罐发酵条件：温度30℃，接种量1%，搅拌转速0~8小时200 r/min，8小时后250 r/min，通风量15~40 L/min，发酵周期30小时，放大罐的发酵工艺：温度30℃，搅拌转速0~8小时130 r/min，8小时后160 r/min，通风量9~15 m³/h，有效活菌数为2.5 × 10⁹/mL。

Abstract: The optimized fermentation medium was obtained by PB design and orthogonal experimental design. Based on fermentation condition in 50L biological reactor, the experiment was scaled up into 400 L fermentor on the basis of same KLa. The results indicated that the living bacteria count of Bacillus amyloliquefaciens was mainly influenced by 、(NH₄)₂SO₄ , cornmeal, corn steep liquor and glucose. The obtained fermentation medium was as follows cornmeal 8 g/L, glucose 12 g/L,(NH₄)₂SO₄ 0.8 g/L, and corn steep liquor 7 g/L. The fermentation condition of 50 L biological reactor was as follows the temperature 30˚C, the inoculation rate 1%, the speed 200 r/min in 0 - 8 hours and 250 r/min after 8 hours, the ventilation quantity 15 - 40 L/min and the fermentation time about 30 hours. The fermentation condition of 400L biological reactor was as follows the temperature 30˚C, the speed 130 r/min in 0 - 8 hours and 160 r/min after 8 hours, the ventilation quantity 9 - 15 m³/h and the fermentation time about 30 hours. Under the condition, the living bacteria count was 2.5 × 10⁹/mL.