硫堇共振瑞利散射光谱法测银离子含量

Determination of Silver Ion by Resonance Rayleigh Scattering Spectrometry with Thionine

摘要: 本文通过共振瑞利散射光谱法研究了硫堇与银离子之间的相互作用。研究表明：在pH = 4.10的B-R缓冲液，20℃ ± 5℃下，t = 20 min，2.0~3.5 mL 2.0 × 10⁻⁴ mol/L 硫堇与银离子存在线性关系。λ = 391 nm时线性关系为ΔIRRS = −5.38 + 44.23C，相关因子r = 0.9990，银离子检出范围ρAg在0.05~5.0 mg/L，检出限ρAg = 0.014 mg/L。硫堇–银分子量增大和疏水界面的生成利于瑞利散射强度增强。

Abstract: In this paper, the interaction between thionine and silver ions was studied by Resonance Rayleigh scattering spectroscopy. Research shows that in the B-R buffer with pH = 4.10, T = 20˚C ± 5˚C, t = 20 min, Vthion = 2.0 - 3.5 mL, c(thion)= 2.0 × 10⁻⁴ mol/L, it has linear relationship between thio and silver ions. λ = 391 nm, linear relationship is ΔIRRS = −5.38 + 44.23C, r = 0.9990, detection range ρAg = 0.05 - 5.0 mg/L, detection limit ρAg =0.014 mg/L. The enhancement of Rayleigh scattering intensity is due to the increase of the molecular weight and the hydrophobic interface of thio-silver.