响应面法优化东莨菪碱的超声提取工艺

Optimization of Ultrasonic Extraction Pro-cess of Scopolamine by Response Surface Methodology

摘要: 本文以洋金花为原料，利用响应面法对超声波辅助二氯甲烷提取东莨菪碱的工艺条件进行优化。在单因素实验的基础上，以液料比、超声功率、超声时间为影响因素，利用Box-Behnken中心组合方法进行3因素3水平实验设计，以东莨菪碱的得率为响应值进行响应面分析。结合实际可操作性，确定最佳提取条件为：液料比11:1，超声功率173 W，超声时间32 min，东莨菪碱的得率为0.09843%，与理论预测值的相对误差为0.53%，说明用响应面法对洋金花中东莨菪碱的超声提取过程优化合理可行。

Abstract: In this paper, the response surface method (RSM) was used to design the experiment, and the ex-tracting process of scopolamine from Flos daturae was optimized by ultrasonic assisted extraction of dichloromethane extraction method. On the basis of the single variable test, the effects of the ratio of material to liquid, ultrasound power and ultrasound time were taken as the influencing factors, the Box-Behnken center combination method was used to design the 3-factor and 3-level experiment, and the response surface analysis was conducted with the scopolamine as the response value. In combination with actual operability, the optimum extraction conditions of scopolamine were determined as follows: the ratio of material to liquid was 11:1, ultrasonic power was 173 W, and ultrasonic time was 32 min. Under this optimum condition, the yield of scopolamine was 0.09843%, and the relative error with the theoretical prediction value was 0.53%, which indicated that it was reasonable and feasible to optimize the extraction process of Scopolamine.