云纹石斑鱼、鞍带石斑鱼与杂交F1的微卫星分析
Microsatellite Analysis of Epinephelus moara, Epinephelus lanceolatus and Hybrid FF1
DOI: 10.12677/HJAS.2018.810169, PDF,  被引量    科研立项经费支持
作者: 李炎璐, 陈 超*：中国水产科学研究院黄海水产研究所，农业部海洋渔业可持续发展重点实验室，青岛市海水鱼类种子工程与生物技术重点实验室，山东 青岛；陈建国, 赵亭亭, 张清雯：中国水产科学研究院黄海水产研究所，农业部海洋渔业可持续发展重点实验室，青岛市海水鱼类种子工程与生物技术重点实验室，山东 青岛;上海海洋大学水产与生命学院，上海；翟介明：莱州明波水产有限公司，山东 烟台
关键词: 云纹石斑鱼；鞍带石斑鱼；杂交F1；微卫星标记；Epinephelus moara； Epinephelus lanceolatus； Hybrid F1； Microsatellite Markers
摘要: 本研究运用微卫星标记分析亲本云纹石斑鱼、鞍带石斑鱼与云纹石斑鱼(♀) × 鞍带石斑鱼(♂)杂交F1的遗传关系，使用13对微卫星引物对3种石斑鱼群体进行微卫星分析，计算等位基因数、基因杂合度、多态信息含量、相似指数和Nei氏遗传距离，并采用UPGMA法构建聚类分析图。结果显示，云纹石斑鱼的平均等位基因数(Ao)最大(5.2900)，鞍带石斑鱼最小(2.6200)；杂交F1的平均观测杂合度(Ho)最高(0.7108)，鞍带石斑鱼最低(0.4167)；13对微卫星引物在云纹石斑鱼、鞍带石斑鱼和杂交F1 3个群体中的平均多态信息含量(PIC)分别为0.4661、0.3392、0.4818；鞍带石斑鱼和杂交F1遗传相似率最高(0.7616)，云纹石斑鱼和鞍带石斑鱼相似率最低(0.4296)。根据遗传距离采用UPGMA法构建聚类分析图，结果显示，鞍带石斑鱼和杂交F1先聚为一支，云纹石斑鱼位于最外支。研究表明，杂交F1群体的遗传多样性水平高于2个亲本群体；杂交F1与鞍带石斑鱼的亲缘关系较近。
Abstract: In this study, the genetic relationship among E. moara, E. lanceolatus and hybrid F1 were analyzed by using 13 microsatellite loci. The allele’s number, observed heterozygosity, expected heterozy gosity, polymorphism information content, genetic similarity index and Nei genetic distances were calculated, and the cluster analysis graph was constructed by UPGMA method. The results showed that the average alleles number of E. moara was the highest (5.2900) and E. lanceolatus was the lowest (2.6200); the average observed heterozy gosity of hybrid F1 was the highest (0.7108) and E. lanceolatus was the lowest (0.4167). Average polymorphic information content by 13 microsatellite primers in the three populations of E. moara, E. lanceolatus and hybrid F1 were 0.4661, 0.3392, 0.4818, respectively. The genetic similarity rate between E. lanceolatus and hybrid F1 was the highest (0.7616), and the lowest was found in E. moara and E. lanceolatus (0.4296). The cluster analysis graph was constructed by UPGMA method according to the genetic distance. The results showed that E. lanceolatus and the hybrid F1 were grouped together, and E. moara was located on the outermost branch. This study showed that the hybrid F1 had a close relationship with E. lanceolatus.
文章引用：李炎璐, 陈超, 陈建国, 赵亭亭, 张清雯, 翟介明. 云纹石斑鱼、鞍带石斑鱼与杂交F1的微卫星分析[J]. 农业科学, 2018, 8(10): 1149-1157. https://doi.org/10.12677/HJAS.2018.810169

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