GRP78蛋白在宫颈组织中表达及与宫颈癌临床特征的关系
Expression of Glucose-Regulated Protein 78 in Cervical Tissue and the Relationship with Clinical Significance
DOI: 10.12677/ACM.2018.89133, PDF,    国家自然科学基金支持
作者: 秦 娟, 林 虹, 刘 卿, 陆安伟：贵州省贵阳市妇幼保健医院妇科，贵州 贵阳
关键词: 宫颈；葡萄糖调节蛋白；HPV分型；Cervical Tissue； Glucose-Regulated Protein； HPV Subtype
摘要: 目的：通过检测葡萄糖调节蛋白78在宫颈癌组织中的表达与临床病理特征的相关性及其与宫颈癌发生、发展的关系。方法：收集贵阳市妇幼保健院妇科宫颈组织标本175例，其中宫颈癌组织89例、宫颈上皮内瘤变组织45例、慢性宫颈炎组织41例。采用免疫组织化学及RT-PCR方法检测GRP78在慢性宫颈炎组织、宫颈上皮内瘤变组织和宫颈癌组织中表达情况。结果：1)慢性宫颈炎组织、宫颈上皮内瘤变及宫颈癌组织中均有GRP78蛋白的表达，GRP78蛋白在宫颈癌组织中阳性表达率显著高于慢性宫颈炎组织及宫颈上皮内瘤变(P < 0.01)；2)宫颈癌患者组织中GRP78的表达与临床病理资料的关系：GRP78在宫颈低分化鳞癌中的表达高于高分化鳞癌(P < 0.05)；GRP78的表达水平随着宫颈癌临床分期较晚而升高；GRP78在宫颈癌有淋巴结转移的患者中表达水平高于无淋巴结转移的患者(P < 0.05)。GRP78蛋白在高危型HPV的宫颈鳞癌组织中的阳性表达率高于低危型HPV的宫颈鳞癌组织(P < 0.05)、HPV阴性宫颈鳞癌组织(P < 0.05)。结论：GRP78在宫颈癌中呈高表达并可能与宫颈癌发生、发展有一定的关系。
Abstract: Objective: To investigate the effects of glucose-regulated protein 78 (GRP78) on the occurrence and development of cervical cancer by detecting the expressions of GRP78 in cervical cancer tissues. Methods: The specimens involving 89 cases of cervical cancer tissues, 45 cases of CIN and 41 cases of chronic cervical tissues were collected from Department of Obstetrics and Gynecology, Guiyang Maternal and Child Health-Care Hospital during January, 2010 to December, 2013. By using immunohistochemistry and RT-PCR techniques, the expressions of GRP78 protein in the cervical cancer tissues, chronic cervical tissues and CIN were detected respectively. Results: 1) GRP78 can be detected in chronic cervical tissues and CIN tissues, and the expression of GRP78 in cervical cancer tissues was obviously higher than that of chronic cervical tissues and CIN tissues (P < 0.01); 2) The expressions of GRP78 in cervical cancer tissues were higher in poorly differentiated squamous cell carcinoma than those in high differentiated squamous cell carcinoma (P < 0.05); The expression of GRP78 in stage II cervical cancer tissues was higher than that in stage I cervical cancer tissues (P < 0.05). The expressions of GRP78 in cervical cancer tissues with lymph node metastasis were higher than those without lymph node metastasis in cervical cancer specimens (P < 0.05). Conclusion: GRP78 protein has higher expression in cervical cancer tissues, and GRP78 might have certain relevance with the occurrence and development of cervical cancer.
文章引用：秦娟, 林虹, 刘卿, 陆安伟. GRP78蛋白在宫颈组织中表达及与宫颈癌临床特征的关系[J]. 临床医学进展, 2018, 8(9): 796-803. https://doi.org/10.12677/ACM.2018.89133

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