白及多糖对PM2.5致小鼠亚急性损伤的防治作用研究
Research on the Prevention and Treatment Effects of Bletilla striata Polysaccharide on Subacute Injury Induced by Repeated Exposure to PM2.5 in Mice
DOI: 10.12677/PI.2018.76024, PDF,    国家自然科学基金支持
作者: 沈颖芝, 姚 玥, 金丽霞, 周芳美*：浙江中医药大学医学技术学院，浙江 杭州；丁兴红, 李美芽：浙江中医药大学基础医学院，浙江 杭州；蒋福升：浙江中医药大学生命科学学院，浙江 杭州
关键词: 白及多糖；PM2.5；亚急性损伤；防治作用；Bletilla striata Polysaccharide； PM2.5； Subacute Injury； Prevention and Treatment Effects
摘要: 目的：探讨白及多糖对PM2.5致小鼠亚急性损伤的防治作用。方法：96只雄性ICR小鼠随机均分为正常组、模型组、白及多糖预保护组、白及多糖治疗组，白及多糖另设低、中、高剂量。采用气管滴注PM2.5悬液的方法建立小鼠亚急性肺损伤模型。预保护组和治疗组分别于造模前后灌胃不同剂量的白及多糖溶液，均持续1周。全自动血细胞分析仪检测小鼠外周血中嗜酸性粒细胞(EOS)、中性粒细胞(NEUT)、单核细胞(MONO)含量；计算比较肺、心脏、脾脏系数变化；光镜下观察小鼠肺组织病理学变化；采用CBA法检测小鼠肺泡灌洗液(BALF)中TNF-α、IFN-γ、MCP-1、IL-6、IL-10、IL-12水平。结果：模型组小鼠全肺系数和脾系数明显升高，BALF中六种细胞因子表达不同程度增强，外周血中EOS、NEUT、MONO分泌增加，差异均有统计学意义(P < 0.01)。白及多糖预保护组和治疗组均能改善小鼠肺组织炎性浸润，调节肺、心脏、脾脏系数趋于正常水平，抑制BALF中免疫因子过度表达，缓解由局部经血液循环引发的全身炎症反应，与模型组比较差异有统计学意义(*P < 0.05, **P < 0.01)。结论：白及多糖能够有效预防和治疗PM2.5相关疾病，与减轻炎症和免疫损伤有关，显示白及抗雾霾具有良好的发展前景。
Abstract: Objective: To investigate the preventive and therapeutic effects of Bletilla striata polysaccharide on subacute injury induced by PM2.5 in mice. Methods: The 96 male ICR mice were randomly divided into normal group, model group, protective group of Bletilla striata polysaccharide, treatment group of Bletilla striata polysaccharide. Bletilla striata polysaccharide groups were set low, medium and high dose. The model of subacute pulmonary injury in mice was established by instilling PM2.5 suspension in tracheal. Protective group and treatment group were gavaged the different doses of Bletilla striata polysaccharide solution before and after the PM2.5 contamination, lasting for one week. The levels of EOS, NEUT, MONO in peripheral blood of mice were detected by automatic hematology analyzer. The changes of lung, heart and spleen coefficient were calculated and compared. The pathological changes of lung tissue were observed with optical microscope. The levels of TNF-α, IFN-γ, MCP-1, IL-6, IL-10 and IL-12 in BALF were detected by CBA. Results: The pulmonary and splenic coefficients of model group increased significantly. To some extent, the expressions of six cytokines in BALF increased and the secretion of EOS, NEUT and MONO in peripheral blood in-creased as well, the differences were significant (P < 0.01). Both protective group and treatment group could ease inflammatory infiltration of lung tissue in mice, regulate the coefficients of lung, heart and spleen tending to the normal level, inhibit the over-expressions of immune factors in BALF and relieve the local to systemic inflammation reaction caused by blood circulation, compared with the model group (*P < 0.05, **P < 0.01). Conclusion: Bletilla striata polysaccharide can prevent and treat PM2.5-interrelated diseases effectively, which is related to the reduction of inflammation and immune damage, indicating that Chinese medicine anti-smog technology has a good prospect.
文章引用：沈颖芝, 姚玥, 丁兴红, 蒋福升, 金丽霞, 李美芽, 周芳美. 白及多糖对PM2.5致小鼠亚急性损伤的防治作用研究[J]. 药物资讯, 2018, 7(6): 144-151. https://doi.org/10.12677/PI.2018.76024

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