光甘草定与酪氨酸酶的相互作用模式研究

Study on the Interaction Mode of Glycyrrhizin and Tyrosinase

摘要: 评价光甘草定对人黑色素瘤细胞内酪氨酸酶活性的影响及探讨其机理，为光甘草定在美白领域的应用提供理论依据。实验借助计算机辅助药物设计软件对光甘草定与酪氨酸酶作用模式进行理论模拟计算，以人黑色素瘤细胞(A375)为模型，采用MTT法测定光甘草定对黑色素细胞活力的影响，基于人酪氨酸酶联免疫试剂盒测定细胞内酪氨酸酶活性。实验结果显示，光甘草定有显著抑制酪氨酸酶活性及抑制黑色素细胞的作用，其作用机制可能是由于与酶活性中心的组氨酸及苯丙氨酸形成相互作用力，从而完美的嵌合在酶活性位点发挥抑制作用。

Abstract: Evaluating the effect and mechanism of Glycyrrhizin on Tyrosinase activity in human melanoma cells is in order to provide theoretical basis for the application of Glycyrrhizin in the field of whitening. The theoretical simulation was carried out by computer-assisted drug design software, and the melanoma cells (A375) were used as the model to determine the whitening effect by MTT assay. Furthermore, we evaluated the Tyrosinase activity by human Tyrosinase enzyme-linked immunoassay kit. The results exhibited that Glycyrrhizin has a significant inhibitory effect on Tyrosinase and melanoma cells. The mechanism may be due to the interaction with Histidine and Phenylalanine in the active center of Tyrosinase, therefore, Glycyrrhizin could be perfectly chimeric at the enzyme active site to exert an inhibitory activity.