养心安神药和重镇安神药对失眠大鼠神经递质的影响
Effects of Yangyin Anshen and Zhongzhen Anshen Chinese Medicine on Neurotransmitters in Insomnia Rats
DOI: 10.12677/TCM.2018.76060, PDF,  被引量    国家自然科学基金支持
作者: 王秀峰, 曾雪爱, 张 瑜, 张一帆, 李 星, 黄俊山：福建省中医药研究院，福建省中医睡眠医学重点实验室，福建 福州
关键词: 失眠；神经递质；安神药；Insomnia； Neurotransmitter； Tranquilizer
摘要: 目的：观察不同类型的安神中药夜交藤、合欢皮、龙骨、牡蛎对失眠大鼠神经递质的影响，探讨它们治疗失眠的药理机制的差异。方法：利用对氯苯丙氨酸(PCPA)建立失眠大鼠模型，造模成功后分别给予夜交藤、合欢皮、龙骨、牡蛎治疗一周。治疗结束后通过酶联免疫吸附测定(Elisa)方法检测大鼠血浆中神经递质γ-氨基丁酸(GABA)、5-羟色胺(5-HT)、多巴胺(DA)和组胺(HIS)的含量。结果：同模型组相比较，夜交藤、合欢皮和龙骨、牡蛎这两类安神药均能够增加失眠大鼠血浆中GABA和5-HT含量(P < 0.01)，同时又能够降低失眠大鼠血浆中DA和HIS的含量(P < 0.01)。结论：夜交藤、合欢皮和龙骨、牡蛎虽然都能使失眠大鼠兴奋性神经递质(DA、HIS)减少，抑制性神经递质(GABA、5-HT)增加，但是夜交藤和合欢皮增加抑制性神经递质作用更显著，而龙骨和牡蛎以降低兴奋性神经递质为主。
Abstract: Objective: To observe the effects of different types of tranquilizers, such as Tuber fleece flower stem, Acacia, Keel, and Oysters on neurotransmitters in insomnia rats, and to explore the differ-ences in their pharmacological mechanisms for treating insomnia. Methods: A rat model of insomnia was established by using p-chlorophenylalanine (PCPA). After successful modeling, it was treated with Tuber fleece flower stem, Acacia, Keel, and Oysters for one week. After treatment, the neurotransmitters γ-aminobutyric acid (GABA), serotonin (5-HT), dopamine (DA) and histamine (HIS) were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the model group, the two kinds of tranquilizers, Tuber fleece flower stem, Acacia, Keel, and Oysters could increase the levels of GABA and 5-HT in the plasma of insomnia rats (P < 0.01), and at the same time they could also reduce the levels of DA and HIS in plasma of insomnia rats (P < 0.01). Conclusion: Evening Tuber fleece flower stem, Acacia, Keel, and Oysters can decrease the levels of excitatory neurotransmitters (DA, HIS) and increase the levels of inhibitory neurotransmitters (GABA, 5-HT) in insomnia rats. But, Tuber fleece flower stem and Acacia increased the inhibitory neurotransmitter effect more significantly, while Keel and Oyster mainly reduced excitatory neurotransmitters.
文章引用：王秀峰, 曾雪爱, 张瑜, 张一帆, 李星, 黄俊山. 养心安神药和重镇安神药对失眠大鼠神经递质的影响[J]. 中医学, 2018, 7(6): 357-363. https://doi.org/10.12677/TCM.2018.76060

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