几种生态除藻方法探索及其应用效果研究
Study on Several Methods of Ecological Algae Removal and Its Application
摘要: 为探索水培条件下生态除藻方法,以霍格兰营养液为对照,采用生菜研究了酵母菌胞外物质,衰老银杏叶片提取液,水葫芦根系分泌物,枯草芽孢杆菌胞外物质对水培生菜容器内藻类的生长抑制和生菜生长的影响。5天后测量,结果表明:在营养液中加入衰老银杏叶片提取液1 ml,营养液的pH和EC值最接近正常范围,枯草芽孢杆菌胞外物质1 ml偏离正常范围最远;在营养液中加入衰老银杏叶片提取液1,2,4 ml或者不加入外来物质,生菜生长最好,枯草芽孢杆菌胞外物质2 ml生菜生长最差;对藻类的抑制效果表现为:水葫芦根系分泌物 > 枯草芽孢杆菌胞外物质 > 酵母菌胞外物质 > 衰老银杏叶片提取液 > 对照组。综合来看,加入衰老银杏叶片提取液1 ml,植株生长情况良好,藻类生长受到一定抑制。
Abstract: To explore ecological algae removal methods under hydroponic conditions, compared with Hoagland nutrient solution, using lettuce, the influence of yeast extracellular substances, aging extracts of Ginkgo leaves, water hyacinth root exudates, Bacillus subtilis extracellular substances on algae growth inhibition and lettuce growth in hydroponic lettuce container was studied. Five days later, the results showed that the pH and EC value of the nutrient solution were close to the normal range by adding 1 ml Ginkgo Biloba Leaves Extract Tablets, and 1 ml extracellular substance of Bacillus subtilis made them deviate most from the normal range. The growth of lettuce was the best in the nutrient solution by adding 1, 2, 4 ml old Ginkgo Biloba Leaves Extract Tablets extract or not adding foreign substances. The growth of lettuce was the worst in the 2 ml extracellular substance of Bacillus subtilis. The inhibitory effects of algae are shown as follows: water hyacinth root exudates > extracellular substance from Bacillus subtilis > yeast extracellular substances > aging extracts of Ginkgo leaf > control group. On the whole, the growth of the plant was in good condition, and the growth of algae was inhibited by the addition of 1 ml Ginkgo Biloba Leaves Extract Tablets.
文章引用:贺忠群, 钟超. 几种生态除藻方法探索及其应用效果研究[J]. 农业科学, 2018, 8(12): 1365-1373. https://doi.org/10.12677/HJAS.2018.812200

参考文献

[1] 沈连静. 水培蔬菜的特点与栽培管理[J]. 吉林蔬菜, 2012, 10(2): 43.
[2] 梁磊. 叶菜类观赏蔬菜的评价及应用研究[D]: [硕士学位论文]. 杭州: 浙江农林大学, 2011.
[3] 丁永良, 张明华, 张建华, 等. 鱼菜共生系统的研究[J]. 中国水产科学, 1997, 4(5): 70-75.
[4] 叶秋雯. 酵母菌对水华藻的竞争性抑制研究[D]: [硕士学位论文]. 上海: 上海交通大学, 2010.
[5] 李合生. 现代植物生理学[M]. 第三版. 北京: 高等教育出版社, 2012.
[6] 陈晓. 小麦叶片中与Rubisco大亚基降解相关的蛋白酶的生化特性、合成定位及分离纯化研究[D]: [硕士学位论文]. 南京: 南京农业大学, 2011.
[7] 朱磊, 胡国梁, 卢剑波, 等. 水葫芦的资源化利用[J]. 浙江农业科学, 2006, 4(4): 460-463.
[8] 张睿, 王广军, 李志斐, 等. 枯草芽孢杆菌对铜绿微囊藻抑制效果的研究[J]. 中国环境科学, 2015, 6(3): 23
[9] 桂英. 水培蔬菜简易栽培技术的研究[D]: [硕士学位论文]. 南京: 南京农业大学, 2009.
[10] 潘杰. 水培生菜技术研究[D]: [硕士学位论文]. 郑州: 河南农业大学, 2003.
[11] 郑悦康, 叶志英, 刘洪波. 5种培养基对酵母菌培养效果的比较[J]. 华南预防医学, 2013, 39(2): 99-100.
[12] 吴蔚, 陈晓, 张鹏, 等. 小麦叶片中1种降解Rubisco大亚基的蛋白酶鉴定[J]. 南京农业大学学报, 2015, 38(1): 63-69.
[13] Trought, M.C.T. and Drew, M.C. (1980) The De-velopment of Satedogging Damage in Wheat Seedling (Triticum wheat L.). I. Shoot and Root Growth in Relation to Changes in the Concentrations of Dissolved Gases and Solutes in the Soil Solution. Plant Soil, 54, 77-94.
[14] 高玲, 丁德荣. 蛋白水解酶在叶细胞中的定位以及与Rubisco降解的关系[J]. 莱阳农学院学报, 1995, 12(4): 268-271.
[15] 郑师章, 何敏. 水葫芦根部分泌物对若干细菌作用的研究[J]. 生态学杂志, 1990, 9(5): 56-57.
[16] 汤保贵, 徐中文, 张金燕, 等. 枯草芽孢杆菌的培养条件及对水质的净化作用[J]. 淡水渔业, 2007, 37(3): 45-48.
[17] 熊庆娥. 植物生理学实验教程[M]. 成都: 四川科学技术出版社, 2015.
[18] 郭世荣. 无土栽培学[M]. 第二版. 北京: 中国农业出版社, 2011.
[19] 罗华建, 郑芝波, 罗诗, 等. 透明花盆水培花卉中藻类抑制技术初探[J]. 亚热带植物科学, 2006(4): 16-18.