钩端螺旋体外膜蛋白Loa22交叉免疫保护作用研究

Cross-Immunoprotectivity of Leptospiral Outer Membrane Protein Loa22

摘要: 研究钩端螺旋体外膜蛋白Loa22免疫血清对不同血清型钩端螺旋体的交叉免疫保护作用。表达纯化钩端螺旋体外膜蛋白Loa22。分别在0、2、4周用纯化蛋白Loa22、LipL32蛋白和PBS经豚鼠皮下多点进行免疫。以LipL32蛋白免疫组为阳性对照，PBS免疫组为阴性对照。末次免疫后2周，用培养7天的不同血清型钩体从腹腔注射攻击豚鼠。连续观察15天，观察各组豚鼠发病情况，并取各组豚鼠的肺、肝、肾作病理切片。分析钩端螺旋体外膜蛋白Loa22对豚鼠的交叉免疫保护作用。蛋白Loa22免疫组的豚鼠用不同血清型钩端螺旋体攻击，无发病现象。豚鼠健康情况良好。而PBS对照组豚鼠出现了厌食、懒动等现象，病理切片有明显的变化。钩端螺旋体外膜蛋白Loa22免疫血清对豚鼠具有交叉免疫保护作用，可以抵抗不同血清型钩体的攻击。

Abstract: The article is to investigate the cross-immunoprotectivity of antisera of Leptospiral outer mem-brane protein Loa22 against various serotypic Leptospira. Leptospiral outer membrane protein Loa22 was expressed and purificated. Guinea pigs were immunized with Loa22 protein, recombinant LipL32 and/or PBS buffer intramuscularly at 2-week interval for three times, respectively, and then challenged with varoius serotypic Leptospira, with recombinant LipL32 as positive control and PBS as negative control. The incidence of immunized-guinea pigs were observed for 15 days. The liver, lung and kindey of immunized-guinea pigs were made into pathlogical slice, with cross-immunoprotectivity of antisera against Leptospiral outer membrane protein Loa22 to guinea pigs analyzed. The guinea pigs immunized with Loa22 protein were challenged with varoius serotypic Leptospira, and they showed resistance to infection but the PBS-immunized guinea pigs not. The liver, lung and kindey of immunized-guinea pigs have changed. The antisera of Leptospiral outer membrane protein Loa22 show good cross-immunoprotectivity to guinea pigs, and can resist infection of various serotypic Leptospira.