两种尿液分析仪和显微镜对尿白细胞检验分析

Analysis of Two Kinds of Urine Analyzer and Microscope for Leucocyte Examination

摘要: 目的：使用尿液干化学分析仪、尿沉渣分析仪与显微镜镜检1042例尿液，对尿液中白细胞结果进行比较，得出尿液中白细胞最为准确的结果。方法：随机选取1042例住院患者尿液标本，分别用尿液干化学分析仪、尿沉渣分析仪、显微镜镜检尿液白细胞(WBC)，对比性分析三个结果。结果：三种方法对尿液中的WBC检出率较一致。1) 尿液干化学分析仪与显微镜镜检对比：WBC敏感性为94.4%，特异性为99.7%，假阴性率18.0%、假阳性率0.7%、总符合率98.8%。2) 尿沉渣分析仪与显微镜镜检对比：WBC敏感性为95.0%，特异性为99.4%，假阴性率5%、假阳性率0.6%、总符合率98.7%。3) 尿液干化学分析仪与尿沉渣分析仪对比：尿干化学分析仪及尿沉渣分析仪检测白细胞结果符合率为98.85%，高度一致(Kappa值为0.993)。P值为0.001，P < 0.05，差异具有统计学意义。结论：尿液干化学分析仪﹑尿沉渣分析仪两种仪器操作简便、快速、检测项目多，重复性好。三种方法检测原理不同导致检验结果存在差异，临床应根据患者的病因和症状，采用多种方法有机结合进行诊断，以提高检测的准确率，为临床提供可靠的检测结果。

Abstract: Objective: The results of white blood cells in 1042 cases of urine were compared by urine dry chemical analyzer, urine sediment analyzer and microscopic examination, and the most accurate results of white blood cells in urine were obtained. Methods: Urine samples of 1042 hospitalized patients were randomly selected, and urine white blood cells (WBC) were detected by urine dry chemical analyzer, urine sediment analyzer and microscopic examination, respectively. Results: The detection rates of WBC in urine by the three methods were consistent. 1) Comparison between urine dry chemical analyzer and microscopy: The sensitivity of WBC was 94.4%, the specificity was 99.7%, the false negative rate was 18.0%, the false positive rate was 0.7%, and the total coincidence rate was 98.8%. 2) Comparison between urine sediment analyzer and microscopy: WBC sensitivity was 95.0%, specificity was 99.4%, false negative rate was 5%, false positive rate was 0.6%, and total coincidence rate was 98.7%. 3) Comparison between urine dry chemical analyzer and urine sediment analyzer: the coincidence rate of white blood cells detected by urine dry chemical analyzer and urine sediment analyzer was 98.85%, highly consistent (Kappa value was 0.993). P value was 0.001, P < 0.05, and the difference was statistically significant. Conclusion: The urine dry chemical analyzer and urine sediment analyzer are simple, rapid and reproducible. Different detection principles of the three methods lead to differences in test results. Clinical diagnosis should be carried out organically by combining multiple methods according to the causes and symptoms of patients, so as to improve the accuracy of test and provide reliable test results for clinical use.