民族资源栘衣多酚氧化酶活性与总酚含量研究

Study on Polyphenol Oxidase (PPO) Activity and Total Phenol Content of Docynia indica

摘要: 采用药食两用植物栘衣嫩枝和叶柄为供试材料，分析植株全年总酚含量和多酚氧化酶(PPO)活性及其变化规律。结果表明，pH 7.0为栘衣植株多酚氧化酶活性的最适值；最佳底物为邻苯二酚，且浓度为0.15 mol•L⁻¹；最佳温度为25℃。栘衣植株总酚含量在每年的4月、5月、9月最低，在花果期栘衣植株多酚氧化酶活性明显高于花前期。

Abstract: Using the twigs and petioles of Docynia indica as test materials, polyphenol oxidase (PPO), total phenol content, and their change pattern were analyzed. The results showed that, the suitable pH value for determining Docynia indica PPO activity was 7.0; the optimal substrate was oxophenic acid with the concentration of 0.15 mol•L⁻¹; the optimum temperature was 25˚C Docynia indica PPO enzyme activity at the flowering and fruiting stage was higher than preflowering stage. The value of Docynia indica total phenol content was minimum in April, May and September in a year.