bFGF-Chitosan通过招募骨髓间充质干细胞加速其向神经细胞分化进程
bFGF-Chitosan Accelerates Its Differentiation into Nerve Cells by Recruiting Bone Marrow Mesenchymal Stem Cells
DOI: 10.12677/HJBM.2019.91004, PDF,    国家自然科学基金支持
作者: 李曼丽*, 李晓光：北京航空航天大学生物医学工程学院生物材料与神经再生北京重点实验室，北京
关键词: 骨髓间充质干细胞；神经球；神经元；bFGF-Chitosan；钙成像； Bone Mesenchymal Stem Cells； Neurosphere； Neuron； bFGF-Chitosan； Calcium Imaging
摘要: 目的：探讨bFGF-chitosan将MSC诱导为神经细胞及其过程。方法：成年大鼠骨髓间充质干细胞分别与bFGF-chitosan和单纯可溶性bFGF共培养，利用nestin，Tuj-1，MAP2等指标分析其分化行为。并利用钙成像技术鉴定其具有神经细胞功能。结果：在诱导19天时在bFGF-chitosan组中能够发现大量神经球样结构，在单纯可溶性bFGF和条件培养基组中能够观察到少量神经球样结构。继续培养能够在bFGF-chitosan组中观察到大量Tuj-1阳性细胞存在并聚集在bFGF-chitosan附近。培养40天时能够观察到MAP2阳性细胞，并能够观察到钙瞬变现象。结论：bFGF-chitosan能够通过将MSC诱导为神经球样结构诱导为具有功能的神经细胞。
Abstract: Objective: To investigate the induction of MSC into nerve cells and its process by bFGF-chitosan. Methods: Adult rat bone marrow mesenchymal stem cells were cultured with bFGF-chitosan and soluble bFGF respectively, and their differentiation behavior was analyzed by means of nestin, Tuj-1 and MAP2. Calcium imaging technique was used to identify its nerve cell function. Results: At 19 days of induction, a large number of neurospheres could be found in the bFGF-chitosan group, and a small amount of neurospheres could be observed in the single soluble bFGF and conditional culture group. Continued culture was able to observe a large number of Tuj-1 positive cells in the bFGF-chitosan group and concentrate near bFGF-chitosan. MAP2 positive cells can be observed at 40 days of culture and calcium transient phenomena can be observed. Conclusion: bFGF-chitosan can induce MSC as a functional nerve cell by inducing it as a neurosphere structure.
文章引用：李曼丽, 李晓光. bFGF-Chitosan通过招募骨髓间充质干细胞加速其向神经细胞分化进程[J]. 生物医学, 2019, 9(1): 23-29. https://doi.org/10.12677/HJBM.2019.91004

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