广谱性拮抗植物病原菌枯草芽孢杆菌的分离鉴定及芽孢形成条件的研究
Isolation of Bacillus subtilis with Broad-Spectrum Antagonism against Plant Pathogens and Its Spore Formation
摘要: 从大兴安岭松苑原始森林林区分离得到的一株对梨黑斑病菌具有拮抗作用的芽孢杆菌，经形态学、生理生化和分子生物学鉴定为枯草芽孢杆菌，命名为Bacillus subtilis Z58；平板对峙实验结果表明B. subtilis Z58对17种植物病原菌均具有拮抗作用；单因素和正交设计得出B. subtilis Z58生成芽孢的最适条件为：发酵液体积：吸附材料质量为4:1，菌体量6 × 109/g和温度为32℃，且该菌株芽孢形成率在15 d达到98%以上，到90 d时基本无营养体存在，该菌株有望开发成农用生防微生物菌剂。
Abstract: A Bacillus strain, isolated from soil of Songyuan (Daxinganling Forest) with antagonism against plant pathogen from pear tree with black spot disease, was identified by morphology, physiology & biochemistry as well as 16 srDNA and designated as Bacillus subtilis Z58. Furthermore, B. subtilis Z58 was confirmed to possess broad-spectrum antagonism against 17 kinds of plant pathogens. Then, parameters for spore formation were designed by both single factors and orthogonal experiment and the results showed the optimal parameters were: fermentation broth: adsorbent of 4:1 (v/m), cell amounts of 6 × 109/g and temperature of 32˚C. Spore proportion kept above 98% from 15 d to 90 d after adsorbing to corncob powder. Thus, B. subtilis Z58 was expected to develop as a biocontrol agent.
文章引用：张戈, 金阳卓越, 崔玉坤, 庞霁岩, 朱瑞艳. 广谱性拮抗植物病原菌枯草芽孢杆菌的分离鉴定及芽孢形成条件的研究[J]. 微生物前沿, 2019, 8(1): 1-8. https://doi.org/10.12677/AMB.2019.81001

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