醛基化玻片固定里默氏杆菌观察生物膜
Observation of Biofilm of RA by Immobilizing on Glutaraldehyde Treated Silicon Surface
DOI: 10.12677/AMB.2019.81002, PDF,    科研立项经费支持
作者: 蒋 姝：重庆轻工职业学院，重庆；重庆科技学院，重庆；廖定容, 王白雪, 陈虹洁, 郭艳婧, 胡梦婷：重庆轻工职业学院，重庆；黄承洪*：重庆科技学院，重庆
关键词: 里默氏杆菌；生物膜；扫描电镜；RA； Biofilm； SEM
摘要: 生物膜是里默氏杆菌(RA)的毒力因子早已被证实，但目前对于RA生物膜的形成机制还研究得不清楚，引起了很多研究者的兴趣。研究目的在于通过对硅基底表面醛基改性处理后固定RA，并研究烘干处理和喷金等处理，直接观察RA的生物膜结构。结果表明醛基化硅表面能有效地固定RA，经60℃烘干处理，喷金5秒可以清楚地观察到RA的生物膜结构，与常规的磷钨酸染色法相比具有耗时短、操作简便等优点，为生物膜进一步研究提供了新的方法学。
Abstract: Biofilm from Riemerella anatipestifer (RA) is a kind of virulence factor that has already been confirmed. However, its formation mechanism and virulence contribution are still not well understood. The purpose of this study is to develop a simple and facile method for BF observation through surface aldehyde immobilization on the silicon substrate and direct investigation of BF microstructure by SEM technique with the help of heating and gold spraying. Results show that RA can be effectively fixed on aldehyde-treated silicon surface. BF microstructure can be clearly observed after 60˚C drying together with 5 seconds gold treatment. Compared with conventional ink staining and phosphotungstic acid staining, this method has the advantages of short time and easy-to-operation. It will provide a novel method for RA’s biofilm research.
文章引用：蒋姝, 廖定容, 王白雪, 黄承洪, 陈虹洁, 郭艳婧, 胡梦婷. 醛基化玻片固定里默氏杆菌观察生物膜[J]. 微生物前沿, 2019, 8(1): 9-14. https://doi.org/10.12677/AMB.2019.81002

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