基于内滤效应的碳量子点荧光探针测定食品中盐酸四环素
Determination of Tetracycline Hydrochloride in Food Samples by Carbon Dots Based on Fluorescence Inner Filter Effects
摘要: 以1-(2-吡啶偶氮)-2-萘酚(PAN)和MnCl2为原料，通过水热法制备了Mn掺杂碳量子点(Mn-CDs)。所制备的Mn-CDs的发射光谱与四环素(TC)的吸收光谱有较好的重叠，当有TC存在的条件下，Mn-CDs的荧光强度(360 nm)被猝灭，且荧光猝灭的程度与TC的浓度(1~200 µM)呈线性关系。TC对Mn-CDs荧光猝灭的机理是内滤作用，所建立的分析方法具有较高的选择性，并成功应用于动物肉样品中TC的测定。
Abstract: In the present work, a selective and sensitive method for detecting tetracycline hydrochloride (TC) using manganese doped carbon quantum dots (Mn-CDs) was developed. The Mn-CDs were prepared via a simple hydrothermal method using 1-(2-pyridinylazo)-2-naphthalenol (PAN) and MnCl2 as precursors. Because of the strong characteristic absorption of TC at 360 nm, which has good spectral overlap with the emission peak of Mn-CDs, the fluorescence intensity of Mn-CDs at 360 nm was linearly quenched in the presence of TC in the concentration range of 1 - 200 µM. The developing assay based on an inner filter effect (IFE) mechanism for detecting TC is selective, convenient, and shows that the as-prepared Mn-CDs have application prospects for simple and specific analytical chemistry.
文章引用：郑彬, 张兰兰, 胡冰倩, 雷雯, 严民民, 柳金枝, 李清媛, 黄飞宏, 孙巍巍, 向国强. 基于内滤效应的碳量子点荧光探针测定食品中盐酸四环素[J]. 自然科学, 2019, 7(2): 72-79. https://doi.org/10.12677/OJNS.2019.72012

参考文献

[1] Tan, H., Ma, C., Song, Y., et al. (2013) Determination of Tetracycline in Milk by Using Nucleotide/Lanthanide Coor-dination Polymer-Based Ternary Complex. Biosensors and Bioelectronics, 50, 447-452. [Google Scholar] [CrossRef] [PubMed]
[2] Huang, X., Liu, C., Li, K., et al. (2013) Occurrence and Distribu-tion of Veterinary Antibiotics and Tetracycline Resistance Genes in Farmland Soils around Swine Feedlots in Fujian Province, China. Environmental Science and Pollution Research, 20, 9066-9074. [Google Scholar] [CrossRef] [PubMed]
[3] (2003) The Code of Federal Regulations, Title 21, Part 556, Sec-tion 152, 500, and 720. US Government Printing Office, Washington DC.
[4] (2009) Commission Regulation (EU) No 37/2010. Official Journal of the European Union L.
[5] Hussien, E.M. (2014) Development and Validation of an HPLC Method for Tetracycline-Related USP Monographs. Biomedical Chromatography, 28, 1278-1283. [Google Scholar] [CrossRef] [PubMed]
[6] Song, J., Zhang, Z.H., Zhang, Y.Q., et al. (2014) Ionic Liquid Dispersive Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography for Determination of Tet-racycline Drugs in Eggs. Analytical Methods, 6, 6459-6466. [Google Scholar] [CrossRef] 
[7] 罗庆, 孙丽娜, 胡筱敏. 固相萃取-高效液相色谱法测定畜禽粪便中罗红霉素和3种四环素类抗生素[J]. 分析试验室, 2014, 33(8): 885-888.
[8] Li, X., Li, C. and Chen, L. (2015) Preparation of Multifunctional Magnetic-Fluorescent Nanocomposites for Analysis of Tetracycline Hydrochloride. New Journal of Chemistry, 39, 9976-9982. [Google Scholar] [CrossRef] 
[9] 檀尊社, 陆恒, 邵伟, 等. 胶体金免疫层析法快速检测水产品中四环素类药物残留[J]. 西北农业学报, 2010, 19(8): 32-37.
[10] 陈玲, 万宇平, 邵兵, 等. 牛奶中β-内酰胺和四环素类抗生素二联检测试纸条的初步研究[J]. 食品与生物技术学报, 2012, 31(7): 776-783.
[11] Choma, I. (2000) TLC Determination of Tetracyclines in Milk. Journal of Planar Chromatography, 13, 261-265.
[12] Zu, F., Yan, F., Bai, Z., et al. (2017) The Quenching of the Fluorescence of Carbon Dots: A Review on Mechanisms and Applications. Micro-chimica Acta, 184, 1899-1914. [Google Scholar] [CrossRef] 
[13] Song-Ling, Y.E., Huang, J.J., Lin, L., et al. (2017) Preparation of Carbon Dots and Their Application in Food Analysis as Signal Probe. Chinese Journal of Analytical Chemistry, 45, 1571-1581. [Google Scholar] [CrossRef] 
[14] Shereema, R.M., Rao, T.P., Sameer Kumar, V.B., et al. (2018) Individual and Simultaneous Electrochemical Determination of Metanil Yellow and Curcumin on Carbon Quantum Dots Based Glassy Carbon Electrode. Materials Science & Engineering C, 93, 21-27. [Google Scholar] [CrossRef] [PubMed]
[15] Mintz, K., Waidely, E., Zhou, Y., et al. (2018) Carbon Dots and Gold Nanoparticles Based Immunoassay for Detection of Alpha-L-Fucosidase. Analytica Chimica Acta, 1041, 114-121. [Google Scholar] [CrossRef] [PubMed]
[16] Shi, W., Li, X. and Ma, H. (2012) A Tunable Ratiometric pH Sensor Based on Carbon Nanodots for the Quantitative Measurement of the Intracellular pH of Whole Cells. Angewandte Chemie-International Edition, 51, 6432-6435. [Google Scholar] [CrossRef] [PubMed]
[17] Wu, P. and Yan, X.P. (2013) Doped Quantum Dots for Chemo/Biosensing and Bioimaging. Chemical Society Reviews, 42, 5489-5521. [Google Scholar] [CrossRef] [PubMed]
[18] Gao, X., Du, C., Zhuang, Z., et al. (2016) Carbon Quantum Dot-Based Nanoprobes for Metal Ion Detection. Journal of Materials Chemistry C, 4, 6927-6945. [Google Scholar] [CrossRef] 
[19] Na, W., Liu, H., Wang, M., et al. (2017) A Boronic Acid Based Glu-cose Assay Based on the Suppression of the Inner Filter Effect of Gold Nanoparticles on the Orange Fluorescence of Graphene Oxide Quantum Dots. Microchimica Acta, 184, 1463-1470. [Google Scholar] [CrossRef] 
[20] Ding, P., Xin, X., Zhao, L., et al. (2017) On-off-On Fluorescent Oligomer as a Chemosensor for the Detection of Manganese (VII), Sulfur (II) and Aldehydes Based on the Inner Filter Effect. RSC Advances, 7, 3051-3058. [Google Scholar] [CrossRef] 
[21] Mao, M., Tian, T., He, Y., et al. (2017) Inner Filter Effect Based Flu-orometric Determination of the Activity of Alkaline Phosphatase by Using Carbon Dots Codoped with Boron and Ni-trogen. Microchimica Acta, 185, 17. [Google Scholar] [CrossRef] [PubMed]
[22] 耿凤华, 王永祥, 丁秀云, 等. 基于内滤效应的信号增强型诺氟沙星荧光分析法[J]. 分析测试学报, 2012, 31(2): 195-199.
[23] Wang, Y., Zhang, Y., Jia, M., et al. (2015) Func-tionalization of Carbonaceous Nanodots from Mn(II)-Coordinating Functional Knots. Chemistry, 21, 14843-14850. [Google Scholar] [CrossRef] [PubMed]
[24] Qun, G. and Kenny, J.E. (2009) Improvement of Inner Filter Effect Correction Based on Determination of Effective Geometric Parameters Using a Conventional Fluorimeter. Analytical Chemistry, 81, 420-426. [Google Scholar] [CrossRef] [PubMed]
[25] Liu, H., Xu, C., Bai, Y., et al. (2017) Interaction between Fluorescein Isothiocyanate and Carbon Dots: Inner Filter Effect and Fluorescence Resonance Energy Transfer. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 171, 311-316. [Google Scholar] [CrossRef] [PubMed]