长白山杜香挥发油提取及功能初步研究
The Preliminary Study on the Extraction and Function of Volatile Oil from Ledum palustre L.
摘要: 本文以长白山宽叶杜香为研究对象，采用水蒸气蒸馏法提取杜香挥发油，利用GC-MS分析挥发油化学组成，并对其抗氧化活性及抑菌活性进行分析。结果：从长白山杜香挥发油中鉴定出32种化学成分，主要成份为β-水芹烯(43.55%)，萜烯醇(11.86%)等，杜香挥发油抗氧化活性为Vc的4.16倍，并对大肠杆菌、金黄葡萄杆菌及枯草芽孢杆菌均具有抑菌作用。结论：杜香中挥发油含量较高，有较强的抗氧化及抑菌活性，可作为天然抗氧化剂和抑菌剂来源。
Abstract: Using steam distillation method, the volatile oil was extracted from Ledum palustre L., its chemical compositions were analyzed by GC-MS. Finally, the antioxidant activity was detected by DPPH scav-enging method, and the antibacterial activity was examined using antibacterial ring and MIC method. Results: 32 chemical constituents were identified from Ledum palustre L. volatile oil of Le-dum palustre L., including beta-Phellandrene (43.55%), 4-methyl-1-(1-methylethyl)-3-Cyclohexen-1-ol (11.86%), and so on. Antioxidant activity of the volatile oil was approximately 4.16 times of Vc. The volatile oil has inhibitory activity to Escherichia coli, Staphylococcus aureus and Bacillus subtilis. Conclusion: The volatile oil is abundant in Ledum palustre L., and has stronger antioxidant and antimicrobial activity. Ledum palustre L. volatile oil can be used as a source of natural antioxidant and bacteriostat.
文章引用：张云飞, 范慧珠, 杨华英, 王丹妮, 王瑞俭, 王金玲. 长白山杜香挥发油提取及功能初步研究[J]. 自然科学, 2019, 7(3): 81-87. https://doi.org/10.12677/OJNS.2019.73013

参考文献

[1] 赵子峰, 沙伟, 赵子云. 我国的杜香属植物及杜香属植物的应用[J]. 生物学教学, 2006, 31(4): 8-10.
[2] Glenn, S.D. (2001) The Rediscovery of Ledum groenlandicum Oeder (Ericaceae) in New Jersey. Journal of the Torrey, Botanical Society, 128, 407-408.
[3] 赵子峰, 沙伟, 赵子云. 我国的杜香属植物及杜香属植物的应用[J]. 生物学教学, 2006, 31(4): 8-9.
[4] 马雪梅. 资源植物杜香的研究进展[J]. 安徽农业科学, 2010, 38(6): 2949-2950.
[5] 姜玮, 刘静波, 卢静, 等. 杜香挥发油研究概述[J]. 食品科学, 2010, 13(3): 337-341.
[6] 江佩芬, 刘建国, 周勇, 等. 杜香植物资源开发利用的基础研究——Ⅱ. 杜香油抗真菌及细菌作用的实验研究[J].天然产物研究与开发, 1993(1): 39-43.
[7] 王炎, 赵敏. 大兴安岭地区细叶杜香挥发性成分的研究[J]. 色谱, 2003(6): 631.
[8] 尤莉艳, 杨婷, 姜辉, 等. 杜香挥发油提取工艺研究及成分GC-MS分析[J]. 江苏农业科学, 2018, 46(10): 1-3.
[9] Ahmoud, A.A. and Ahmed, A.A. (2006) α-Pinene-Type Monoterpenes and Other Constituents from Artemisia suksdorfii. Phytochemistry, 67, 2103-2109. [Google Scholar] [CrossRef] [PubMed]
[10] Belousova, N.V., Khan, V.A. and Berezovskaya, T.P. (1990) Intraspecies Chemical Variability of the Essential Oil of Ledum palustre. Chemistry of Natural Com-pounds, 26, 398-405. [Google Scholar] [CrossRef] 
[11] Gretsusnikova, T., Jarvan, K., Orav, A., et al. (2010) Compara-tive Analysis of the Composistion of the Essential Oil from the Shoots, Leaves and Stems the Wild Ledum palustre L. from Estonia. Procedia Chemistry, 2, 168-173. [Google Scholar] [CrossRef] 
[12] 缪成贵, 陈庆榆, 何华奇. 采用DPPH法测定四大药用名菊抗氧化活性[J]. 安徽科技学院学报, 2012, 26(4): 39-42.