甘草次酸修饰的金属有机框架载药系统的研究
Study on Metal-Organic Framework Drug Loading System Modified by Glycyrrhetinic Acid
DOI: 10.12677/PI.2019.83008, PDF,    科研立项经费支持
作者: 杨 春, 赵僧群, 韩莎莎：辽宁大学药学院，辽宁 沈阳；李 丽*：辽宁大学药学院，辽宁 沈阳;辽宁大学司法鉴定研究院，辽宁 沈阳
关键词: 金属有机框架材料；甘草次酸；肝靶向性；抗肿瘤活性；Metal Organic Frame； Glycyrrhetinic Acid； Liver Targeting； Anticancer Effect
摘要: 目的：以金属有机框架材料UiO-66-NH2为载体，以甘草次酸(GA)对其进行表面化学修饰，构建UiO-66- NH2-GA，考察其作为药物载体的载药能力。方法：以一锅法合成载体UiO-66-NH2，在UiO-66-NH2上修饰GA，以浸渍法制备装载5-FU的5-FU@UiO-66-NH2-GA，通过X射线衍射法(XRD)、红外光谱法(IR)、差示扫描量热法(DSC)和扫描电镜(SEM)对UiO-66-NH2-GA及5-FU@UiO-66-NH2-GA的晶型结构及载药性能进行表征，通过MTT实验研究5-FU@UiO-66-NH2-GA的体外抗肿瘤活性。结果：UiO-66-NH2及UiO-66-NH2-GA均为正八面体结构且晶型结构稳定，UiO-66-NH2-GA对5-FU的载药率高达80.9%，5-FU@UiO-66-NH2-GA在pH 5.5的释放介质中比pH 6.5，7.4可以更好地有效释放，5-FU@UiO-66-NH2-GA比5-FU具有更强的抑制肝癌细胞生长的能力。结论：本研究成功制备出具有良好肝靶向性的药物载体UiO-66-NH2-GA，为金属有机框架作为药物载体提供有力的依据。
Abstract: Objective: The purpose of this study was to formulate and evaluate the metal organic framework modified with glycyrrhetinic acid (UiO-66-NH2-GA). Methods: The metal organic framework (UiO-66-NH2) was prepared via one pad method, and modified by glycyrrhetinic acid. 5-FU@UiO- 66-NH2-GA was generated by impregnation method. It was characterized by X-ray diffraction (XRD), infrared spectroscopy (IR), differential scanning calorimetry (DSC) and scanning electron micros-copy (SEM). The loading capacity, release capacity, and anticancer effect were also investigated. Results: The characterization experiments indicated that UiO-66-NH2 and UiO-66-NH2-GA were both regular octahedral structure and the crystal structure were stable. The drug loading reached 80.90%. 5-FU@UiO-66-NH2-GA showed a higher cumulative release rate at pH 5.5 than that at pH 6.5 and 7.4. The results from MTT suggested that UiO-66-NH2-GA significantly improved the anti-cancer effect of 5-FU. Conclusion: This paper provided a powerful basis for UiO-66-NH2-GA as a good drug carrier.
文章引用：杨春, 赵僧群, 韩莎莎, 李丽. 甘草次酸修饰的金属有机框架载药系统的研究[J]. 药物资讯, 2019, 8(3): 62-72. https://doi.org/10.12677/PI.2019.83008

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