偶氮二醇烯鎓功能化等离子体聚烯丙胺涂层制备及其抗凝血研究
Plasma Polymerization of Allylamine Coating for Loading Nitric Oxide and the Resulting Effects on Antithrombogenicity
DOI: 10.12677/MS.2019.94054, PDF,    科研立项经费支持
作者: 杜泽煜：西南交通大学，生命科学与工程学院，四川 成都；田文杰：四川省人民医院和四川省医学科学院心内科，四川 成都；卢 静：四川省人民医院和四川省医学科学院麻醉科，四川 成都；黄 楠：西南交通大学，材料先进技术教育部重点实验室，四川 成都
关键词: 偶氮二醇烯鎓；等离子体聚合；烯丙胺；抗凝血；NONOate； Plasma Polymerization； Allylamine； Anticoagulation
摘要: 偶氮二醇烯鎓(NONOate)在生理条件下可以质子化水解，释放具有生物活性的一氧化氮(NO)分子，从而可赋予偶氮二醇烯鎓化器材表面特殊的生理功能及改善材料表面的血液相容性。本研究利用等离子体聚烯丙胺(PPAam)技术在316 L不锈钢(SS)表面构建富氨基涂层，并进一步在碱性醇溶液利用NO与涂层中丰富仲氨基结合将PPAam偶氮二醇烯鎓化，最终得到具有NO释放能力的抗凝血NO-PPAam涂层。通过傅立叶变换红外光谱(FTIR)和X射线光电子能谱(XPS)结果证实NO成功固定到PPAam中。实时化学发光法(Chemiluminescence)测得NO-PPAam涂层可释放NO量达到41.5 nmol/cm2。体外血小板实验证实了NO-PPAam涂层通过释放NO显著抑制了血小板的粘附激活，进一步在半体内血液循环试验中体现出了优异的抗凝血性能。因此，该方法构建的具有高效NO释放能力的NO-PPAam涂层对于提高血液接触类器材表面血液相容性具有潜在的应用前景。
Abstract: Diazeniumdiolates (NONOate) can be formed and be anchored to secondary amino (SA) via the reaction between nitric oxide (NO) and SA, and can be protonized in physiological fluid to release nitric oxide (NO) molecules, which has various biological functions, especially the anticoagulation properties. In this study, NO was incorporated in a secondary-amino rich plasma polymerized allyl amine coating (marked as NO-PPAam) by forming the NONOate under alkaline alcohol solution. The successful produce of NONOate was confirmed by the fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). The cumulative NO release from NO-PPAam was detected by chemiluminescence and was up to 41.5 nmol/cm2. In vitro experiments showed that the NO re-leased from NO-PPAam coating significantly inhibited the platelets adhesion and activation on this coating. Extracorporeal circulation test revealed that the NO-PPAam coating could dramatically reduce thrombus compared to PPAam and 316 L SS. The strategy of constructing a NO-PPAam coating on substrate shows a promising way to improve the hemocompatibility of blood-contacting devices.
文章引用：杜泽煜, 田文杰, 卢静, 黄楠. 偶氮二醇烯鎓功能化等离子体聚烯丙胺涂层制备及其抗凝血研究[J]. 材料科学, 2019, 9(4): 418-427. https://doi.org/10.12677/MS.2019.94054

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