纳米金表面增强拉曼光谱法快速检测雌二醇
Rapid Determination of Estradiol by Nano-Gold Surface-Enhanced RamanSpectroscopy
摘要: 采用纳米金表面增强拉曼光谱法，建立快速检测雌二醇的方法。利用单因素分析法确定了纳米金、雌二醇和硝酸溶液的最优加入量以及拉曼光谱的较佳采集时间，结合拉曼谱图对雌二醇表面增强拉曼谱图中的特征峰进行了归属，从而建立雌二醇快速检测的定性定量分析模型。以雌二醇浓度与915.4 cm−1处特征峰峰强建立标准曲线方程，可达到0.04 μg•mL−1的最低检测浓度。该方法反应、测试时间只需30 s，平均回收率在90%~110%。该方法有望应用于环境中雌二醇残留的快速检测。
Abstract: A rapid method for the determination of estradiol was developed by nano-gold surface-enhanced Raman spectroscopy. The optimal addition amount of nano gold, estradiol and nitric acid solution as well as the better acquisition time of Raman spectra were determined by single factor analysis method. Combined with Raman spectra, the characteristic peaks in the surface enhanced Raman spectra of estradiol were attributed. Thus, a qualitative and quantitative analysis model for the rapid determination of estradiol was established. The standard curve equation was established based on the estradiol concentration and the characteristic peak strength at 915.4 cm−1, which could reach the minimum detection concentration of 0.04 μg∙mL−1. The reaction and test time of this method is only 30 s, and the average recovery is 90%-110%. This method is expected to be applied to the rapid detection of estradiol residues in the environment.
文章引用：康崇伟, 宋奇凡, 左成明, 宋庚益, 陈佳佳, 周亚红. 纳米金表面增强拉曼光谱法快速检测雌二醇[J]. 分析化学进展, 2019, 9(2): 61-70. https://doi.org/10.12677/AAC.2019.92009

参考文献

[1] Chang, H.S., Choo, K.H., Lee, B. and Choi, S.J. (2009) The Methods of Identification, Analysis, and Removal of Endocrine Disrupting Compounds (EDCs) in Water. Journal of Hazardous Materials, 172, 1-12.
[Google Scholar] [CrossRef] [PubMed]
[2] Sun, M.M., Du, L.Y., Gao, S.Q., Bao, Y.H. and Wang, S.H. (2010) Deter-mination of 17β-Oestradiol by Fluorescence Immunoassay with Streptavidin-Conjugated Quantum Dots as Label. Steroids, 75, 400-403.
[Google Scholar] [CrossRef] [PubMed]
[3] Ying, G.G., Kookana, R.S. and Ru, Y.J. (2002) Occurrence and Fate of Hormone Steriods in the Environment. Environment International, 28, 545-551.
[Google Scholar] [CrossRef] [PubMed]
[4] Quintana, J.B., Carpinteiro, J., Rodriguez, I., et al. (2004) Determination of Natural and Synthetic Estrogens in Water by Gas Chromatography with Mass Spectrometric Detection. Journal of Chromatography A, 1024, 177-185.
[Google Scholar] [CrossRef] [PubMed]
[5] 廖涛, 吴晓翠, 王少华, 等. 固相萃取——气相色谱/质谱联用法同时检测水体中9种环境雌激素[J]. 分析化学, 2013, 41(3): 422-426.
[6] Yan, W., Zhao, L.X., Feng, Q.Z., Wei, Y.L. and Lin, J.M. (2009) Simultaneous Determination of Ten Estrogens and Their Metabolites in Waters by Improved Two-Step SPE Followed by LC-MS. Chromatographia, 69, 621-628.
[Google Scholar] [CrossRef] 
[7] Guo, F., Liu, Q., Qu, G.B., et al. (2013) Simultaneous Determination of Five Estrogens and Four Androgens in Water Samples by Online Solid-Phase Extraction Coupled with High-Performance Liquid Chro-matography-Tandem Mass Spectrometry. Journal of Chromatography A, 1281, 9-18.
[Google Scholar] [CrossRef] [PubMed]
[8] Chen, L. and Choo, J. (2008) Recent Advances in Surface-Enhanced Raman Scattering Detection Technology for Microfluidic Chips. Electrophoresis, 29, 1815-1828.
[Google Scholar] [CrossRef] [PubMed]
[9] Park, H., Lee, S., Chen, L., et al. (2009) SERS Imaging of HER2-Overexpressed MCF7 Cells Using Antibody-Conjμgated Gold Nanorods. Physical Chemistry Chemical Physics, 11, 7444-7449.
[10] Bell, S.E.J. and Sirimuthu, N.M.S. (2006) Surface-Enhanced Raman Spectroscopy (SERS) for Sub-Micromolar Detection of DNA/RNA Mono-nucleotides. Journal of the American Chemical Society, 128, 15580-15581.
[Google Scholar] [CrossRef] [PubMed]
[11] Barhoumi, A. and Halas, N.J. (2010) Label-Free Detection of DNA Hybridization Using Surface Enhanced Raman Spectroscopy. Journal of the American Chemical Society, 132, 12792-12793.
[Google Scholar] [CrossRef] [PubMed]
[12] Sha, M.Y., Xu, H., Penn, S.G., et al. (2007) SERS Nanoparticles: A New Optical De-tection Modality for Cancer Diagnosis. Nanomedicine, 2, 725-734.
[Google Scholar] [CrossRef] [PubMed]
[13] 欧阳雨. 乐果涂膜表面增强拉曼光谱研究[J]. 分析测试学报, 2012, 32(8): 996-1000.
[14] Mosier-Boss, P.A., Lieberman, S.H. (2003) Sur-face-Enhanced Raman Spectroscopy (SERS) and Molecular Modeling of the Chromate Interaction with 4-(2-Mercaptoethyl)Pyridinium. Langmuir, 19, 6826-6836.
[Google Scholar] [CrossRef] 
[15] 牛书操, 吕珍珍, 刘金钏, 蒋小玲, 李诚, 陈爱亮. 基于适配体的纳米金比色法快速检测雌二醇研究[J]. 分析测试学报, 2014(7): 835-839.
[16] Frens, G. (1973) Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions. Nature Physical Science, 241, 20-22.
[Google Scholar] [CrossRef] 
[17] Turkevich, J., Stevenson, P.C. and Hillier, J. (1951) A Study of the Nucleation and Growth Processes in the Synthesis of Colloidal Gold. Discussions of the Faraday Society, 11, 55-75.
[Google Scholar] [CrossRef] 
[18] 陶进江, 潘桂根, 刘木华, 袁海超, 赵进辉. 基于表面增强拉曼光谱的鸭肉中己烯雌酚残留检测[J/OL]. 食品与机械, 2018(12): 3-11.
[19] 王乐, 张爱霞, 陈晞, 等. 表面增强拉曼光谱法快速检测饮料中玫瑰红B[J]. 食品研究与开发, 2018, 39(7): 126-130.
[20] 高书燕, 张树霞, 杨恕霞, 等. 表面增强拉曼散射活性基底[J]. 化学通报, 2007, 70(12): 908-914.
[21] 张亚南. 多吡啶铜配合物的合成及与 DNA 的相互作用研究[D]: [硕士学位论文]. 新乡: 河南师范大学, 2013: 38-51.

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