对比槲皮素在正常饮食和高脂饮食条件下对小鼠肝脏基因调节的异同

Comparison of the Similarities and Differences of Quercetin on Gene Regulation in Mice Liver under Normal Diet and High-Fat Diet

摘要: 目的：对比0.05%剂量时槲皮素在正常和高脂两种饮食条件下对小鼠肝脏基因调节的异同。方法：在NIH-Geo数据库中找到槲皮素干预小鼠肝组织基因表达的两个微阵列数据库GSE62805和GSE38141，运用GEO2R的分析方法分析槲皮素对小鼠肝脏基因表达谱的影响，选择有显著性差异(p < 0.05)并且差异倍数大于2或者小于0.5的基因进行分析对比。并用David数据库对两个数据库中槲皮素干预后差异基因表达趋势相同(均表现为上调或均表现为下调)以及表达趋势相反的基因进行GO富集分析。结果：饲料中添加0.05%的槲皮素饲喂小鼠20周，在正常饮食和高脂饮食两种条件下对小鼠肝脏基因的影响存在异同。1) 槲皮素在两种饮食条件下对小鼠肝脏基因表达谱的影响有40个基因调节趋势一致，其中有28个基因在两种饮食条件下都表现为上调，12个基因都表现为下调。2) 槲皮素在两种饮食条件下对小鼠肝脏有48个基因调节趋势相反，其中有11个基因在正常饮食条件下都表现为上调，在高脂饮食条件下表现为下调；另有37个基因在正常饮食条件下都表现为下调，而在高脂饮食条件下表现为上调。3) 槲皮素在两种饮食条件下对小鼠肝脏调节趋势一致的40个基因，其调节程度并不相同。4) GO富集分析发现表达趋势一致的基因参与了细胞分泌、突触形成和细胞间信号传导，表达趋势相反的基因与细胞质膜、突触和细胞连接相关。结论：槲皮素对小鼠肝脏基因的调节与小鼠的机体状态有关，不同机体状态其调节作用存在差异。

Abstract: Objective: Compare the similarities and differences of quercetin on gene regulation in mice liver under normal diet and high-fat diet. Method: Searching for the GSE62805 and GSE38141 which analyze the gene expression in liver of mice under normal diet or high-fat diet after treated with quercetin in NIH-Geo database. And analyze the data by GEO2R selecting the genes whose fold change are more than 2 or less than 0.5 as the regulated gene. Using David to conduct GO analysis for regulated genes, the regulated genes which are upregulated or downregulated simultaneously in both datasets as a group, the regulated genes which are upregulated in one dataset and down-regulated in another datasets as the other group. Results: There are similarities and differences of quercetin on gene regulation in liver of mice under normal diet and high-fat diet. 1) There are 40 regulated genes which are upregulated or downregulated simultaneously in both datasets, includ-ing 28 upregulated genes and 12 downregulated genes. 2) There are 48 regulated genes which present opposite expression trend, including 11 regulated genes which are upregulated under normal diet but downregulated under high fat diet and 37 regulated genes which are downregulated under normal diet but upregulated under high fat diet. 3) The regulatory degree of quercetin on 40 regulated genes which present same trend in both datasets is different. 4) The 40 regulated genes which present same expression trend in both datasets are involved in synapse, secretion by cell and generation of cell-cell signaling and the 48 regulated genes which present opposite expression trend related to plasma membrane part, synapse and cell junction. Conclusion: The regulation of quercetin on gene expression in liver of mice is related to body state. The regulation is different in different body states.