高效液相色谱法同时测定川芎茶调丸中升麻素苷和5-O-甲基维斯阿米醇苷的含量

Simultaneous Determination of Prim-O-Glucosylcimifugin and 5-O-Methylvisammioside in Chuanxiongchatiao Pills by HPLC

摘要: 目的：建立HPLC法同时测定川芎茶调丸中升麻素苷和5-O-甲基维斯阿米醇苷含量的方法。方法：采用HPLC法，色谱柱为Agilent Eclipse XDB-C18；水为流动相A，乙腈为流动相B，梯度洗脱；流速为1 mL/min；检测波长为302 nm。结果：升麻素苷、5-O-甲基维斯阿米醇苷分别在0.0996~0.996 µg (r = 0.9999)和0.1428~1.428 µg (r = 0.9998)之间线性关系良好。升麻素苷、5-O-甲基维斯阿米醇苷平均回收率分别为97.89% (RSD = 2.33%)和97.40% (RSD = 1.43)。结论：该法稳定、准确，重现性好，阴性对照无干扰，可用于川芎茶调丸中色原酮类成分的含量测定。

Abstract: Objective: To establish a HPLC method for the Determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside in Chuanxiongchatiao pills. Methods: The HPLC analysis was carried out on Agilent Eclipse XDB-C₁₈ (250 × 4.6 mm, 5 μm) with a mixture of acentonitrile-water as the mobile phase. The determination wavelength was set at 302 nm with the flow rate of 1.0 mL/min. Results: The prim-O-glucosylcimifugin and 5-O-methylvisammioside showed good linearity in the range of 0.0996 - 0.996 µg (r = 0.9999) and 0.1428 - 1.428 µg (r = 0.9998) respectively. The average recovery rates were 97.57% (RSD = 1.16) and 97.74% (RSD = 1.26) respectively. Conclusions: The method is stable and accurate. It can be used for the content determination of prim-O-gluco- sylcimifugin and 5-O-methylvisammioside in Chuanxiongchatiao pills.