WOX5过量表达和秋水仙素处理对拟南芥根冠柱细胞与侧根冠细胞的影响差异

Differential Impacts of WOX5 Overexpression and Colchicine Treatments on Columella and Lateral Root Cap Cells in Arabidopsis thaliana

摘要: 柱细胞和侧根冠细胞为植物根冠中的两种细胞类型，它们虽然来自不同的干细胞，但分裂与分化活动高度同步。WOX5为拟南芥中根尖干细胞调控的一个重要基因，其所编码的蛋白对根冠细胞有抑制分化和促进脱分化的功能，但这些功能之间是否相互依赖目前并不清楚。本研究利用秋水仙素处理WOX5过表达幼苗后，发现WOX5对脱分化的促进作用不依赖于细胞分裂，且在WOX5过量表达的背景下，柱细胞和侧根冠细胞间对秋水仙素处理呈现出显著差异。研究结果为准确了解WOX5的调控功能提供了新的实验数据，为研究植物细胞间相互协调提供了一个重要切入点和研究思路。

Abstract: Columella and lateral root cap cells are two different cell types in root cap of higher plants. Al-though they are generated by different stem cells, their division and differentiation are highly coordinated and synchronised. WOX5 is an important regulatory gene for root stem cells in Ara-bidopsis thaliana and its encoded product can inhibit the differentiation and also promote dedif-ferentiation of root cap cells, but the relationship between these two functions is currently unclear. In this study, we used colchicine to treat WOX5-overexpressing seedlings and found that the dedifferentiation-promoting function of WOX5 did not depend on cell division. In addition, in the background of WOX5-overexpression, columella and lateral root cap cells displayed differential responses to colchicine treatments. The results provide new experimental data for the regulatory function of WOX5 and also an important entry point and experimental strategy for studying the coordinations of plant cells.