天女木兰组培污染菌的鉴定及多样性分析
Identification and Diversity Analysis of Contaminant Bacteria in Tissue Culture of Magnolia sieboldii
摘要: 采用划线分离法从天女木兰外植体培养基中分离污染菌株，用16S rDNA序列对污染菌株进行核酸分类学鉴定，运用MEGA7.0软件中NJ树法对天女木兰组培污染菌进行聚类分析。结果：从天女木兰组培培养基中分离出95株污染菌，天女木兰污染菌Shannon指数(He’)值为1.91，存在明显优势菌群，绝大多数为革兰氏阴性菌。污染菌大多数在进化上较为接近。结论：天女木兰组培污染菌多样性较低，以革兰氏阴性菌为主，在污染菌控制上可重点参考革兰氏阴性菌的抑菌方法。
Abstract: Contaminant bacteria were isolated from the tissue culture medium of Magnolia sieboldii using streak plate method. Strains were identified using 16S rDNA sequencing analysis. Then cluster analysis was performed by NJ tree method in MEGA7.0 software. Results: 95 strains were isolated from the tissue culture medium of Magnolia sieboldi. Shannon index (He’) of flora is 1.91. Most strains were gram-negative bacteria. Those strains were close in evolution relationship. Conclusion: The diversity of contamination bacteria of Magnolia sieboldii was lower, most belongs to gram-negative bacteria. So the control strategy of gram-negative bacteria could be used in con-taminant of Magnolia sieboldii tissue culture.
文章引用：郑小平, 唐薇, 尹靖琨, 马一宁, 陈露, 杨影, 王瑞俭, 王欢. 天女木兰组培污染菌的鉴定及多样性分析[J]. 自然科学, 2019, 7(3): 153-158. https://doi.org/10.12677/OJNS.2019.73022

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