应用超高效液相色谱串联三重四极杆飞行时间质谱对多种新型毒品的定量定性测定

Qualitative and Quantitative Analysis of Various New Psychoactive Substancesby UPLC-QTOF-MS/MS

摘要: 本文建立了高效液相色谱串联四极杆飞行时间质谱(HPLC-QTOF-MS)同时检测多种新型毒品的定性定量方法，并对方法进行了验证。结果显示待测的二十四种毒品中，DL-MEH、4-MMH、4-MMC、4-MEC、MDPV、ETA、RCS-4、BK-MBDB、2C-C、2C-B、2C-E、AM2201在40 ng•mL⁻¹~1000 ng•mL⁻¹的范围内线性良好，MAM-2201、JWH-073、JWH-018、JWH-019、AM694、PB-22、JWH-250、JWH-203、JWH-307、UR-144、JWH-122在4 ng•mL−1~1000 ng•mL−1的范围内线性良好，JWH-210在1 ng•mL⁻¹~1000ng•mL⁻¹的范围内线性良好(相关系数均大于0.99)，23种新型毒品的检出限在0.2 ng•mL⁻¹~40 ng•mL⁻¹之间，定量限在2 ng•mL⁻¹~200 ng•mL⁻¹之间，回收率在90%~110%之间，精密度均小于5%。此方法拥有操作简单、自动化程度高、准确度高、灵敏度高及检测成本低优点，适用于实际案件中对新型毒品进行定性定量的检测。

Abstract: A high performance liquid chromatography series quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method was developed for the simultaneous determination of various new psy-choactive substances. The method was validated. The results showed that the twelve kinds of DL-MEH, 4-MMH, 4-MMC, 4-MEC, MDPV, ETA, RCS-4, BK-MBDB, 2C-C, 2C-B, 2C-E and AM2201 were in the range of 40 ng•mL⁻¹ - 1 000ng•mL⁻¹ with good linearity, MAM-2201, JWH-073, JWH-018, JWH-019, AM694, PB-22, JWH-250, JWH-203, JWH-307, UR-144 and JWH-122 were in the range of 4 ng•mL⁻¹ - 1000 ng•mL⁻¹ with good linearity, JWH-210 was in the range of 1 - 1000 ng•mL⁻¹ with good linearity (the correlation coefficients were more than 0.99); the detection limit of 23 new drugs was 0.2 ng•mL⁻¹ - 40 ng•mL⁻¹; quantitative limit 2 ng•mL⁻¹ - 200 ng•mL−¹; recovery rate 90% - 110%; the precision is less than 5%. This method was simple, automation, high accuracy, sensitivity and low detection cost. It is suitable for the qualitative and quantitative analysis for the new drugs in practical cases.