野巴子粗多糖的提取及生物活性测定

Extraction and Bioactivity Determination of Polysaccharide from Elsholtzia rugulosa Hemsl.

摘要: 采用超声波辅助水提醇沉法从野巴子叶中提取野巴子粗多糖(YBZc)，野巴子多糖的提取率为5.63%，硫酸–咔唑法测得其糖醛酸含量为32.2%，凯氏定氮法测得蛋白质含量为2.98%。气相色谱分析结果表明YBZc的单糖组成为Rha、Ara、Man、Glc、Gal，相对摩尔比为1.000:0.793:0.440:0.557:0.994。YBZc的活性实验表明，在浓度达到3.2 mg/mL时，YBZc对∙OH、DPPH∙的清除率最大分别为50.14%、97.37%，且在一定的范围内存在量效关系；在浓度达到1.0 mg/mL时，YBZc对α-葡萄糖苷酶抑制效果最佳，为90.28%，且在一定的范围内存在量效关系。表明YBZc具有一定的清除∙OH和DPPH∙的活性以及降血糖效果。YBZc能减弱STS介导的乳鼠海马神经元损伤，具有对海马神经细胞的保护作用。

Abstract: Ultrasound-assisted water extraction and alcohol precipitation method was used to extract the polysaccharide from Elsholtzia rugulosa Hemsl. (YBZc), the extraction rate is 5.63%. The uronic acid content was determined by the sulfuric acid-carbazole method to be 32.2%, and the protein content measured by the Kjeldahl method was 2.98%. The results of gas chromatography showed that the monosaccharide composition of YBZc was Rha, Ara, Man, Glc, Gal, and the relative molar ratio was 1.000:0.793:0.440:0.557:0.994. When the concentration reached 3.2 mg/mL, the maximum clearance rate of YBZc to ∙OH and DPPH∙ was 50.14% and 97.37%, respectively, and there was a dose-effect relationship in a certain range; when the concentration reached 1 mg/mL, YBZc had the best inhibitory effect on α-glucosidase, which was 90.28%, and there is a dose-effect relationship within a certain range. It indicates that YBZc has certain activities of clearing ∙OH and DPPH∙ and hypoglycemic effects. YBZc can attenuate STS-mediated hippocampal neuronal damage in rats and has protective effects on hippocampal neurons.