插穗年龄对毛白杨不定根形成影响的转录组分析
Transcriptome Analysis of the Effect of Cutting Age on Adventitious Root Formation of Populus
DOI: 10.12677/BR.2019.83038, PDF,  被引量    科研立项经费支持
作者: 田知秋, 洪汉辉, 康向阳, 汪晓峰, 薛 华：北京林业大学，北京林业大学生物科学与技术学院，林木育种国家工程实验室，北京
关键词: 杨树插穗；成熟效应；不定根；转录组；Populus Cuttings； Age Effect； Adventitious Roots； Transcriptome
摘要: 为了探究插穗年龄对不定根生根的影响，本研究以1、2、3年生毛白杨杂交三倍体新品种“北林雄株2号”的嫩枝插穗为研究材料，扦插五天后进行转录组测序分析，共检测到5153个差异表达基因。GO分析显示，上调基因主要富集在线粒体呼吸、氧化磷酸化和类黄酮合成等过程，下调基因主要富集在油菜素内酯合成、水杨酸信号转导和赤霉素合成等途径。KEGG结果显示，差异基因在信号转导、能量代谢、翻译、糖代谢、其他次生代谢产物合成、氨基酸代谢和运输等通路富集。最后，对三个比较组中5个共下调基因和4个共上调基因与不定根形成的关系进行了讨论。转录组分析说明随着母株年龄的增长，不定根形成相关基因表达谱发生变化，导致插穗不定根生根受到抑制。
Abstract: In order to explore the effect of mother plant age on adventitious rooting, the five-day-old stem cuttings of a 1-, 2- and 3-year-old hybrid triploid cultivar Populus “Beilinxiongzhu 2” were used as materials for transcriptome sequencing analysis. Significant differentially expressed genes were screened, and GO enrichment analysis and KEGG enrichment analysis were carried out for differentially expressed genes. A total of 5153 differentially expressed genes were identified. Through GO analysis of three comparative groups, the up-regulated genes were mainly concentrated in mitochondrial respiration, oxidative phosphorylation and flavonoid biosynthesis. The down-regulated genes were mainly concentrated in the synthesis of brassinolide, salicylic acid-mediated signaling pathway and gibberellin biosynthesis. KEGG analysis showed that differentially expressed genes were enriched in signal transduction, energy metabolism, translation, sugar metabolism, biosynthesis of other secondary metabolites, amino acid metabolism and transportation. Finally, the relationship between adventitious root formation with 5 down-regulated genes and 4 up-regulated genes was discussed. The transcriptome analysis showed that the expression profiles of adventitious root formation related genes changed with increased age of the mother plant, which led to the inhibition of adventitious root formation in Populus cuttings.
文章引用：田知秋, 洪汉辉, 康向阳, 汪晓峰, 薛华. 插穗年龄对毛白杨不定根形成影响的转录组分析[J]. 植物学研究, 2019, 8(3): 293-306. https://doi.org/10.12677/BR.2019.83038

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