蒙药伊赫汤抑制高糖引起的人腹膜间皮细胞炎症反应和纤维化的体外实验研究

Inhibitory Effect of Mongolian Medicine Yihe Decoction on Inflammatory Reaction and Fibrosis of Human Peritoneal Mesothelial Cells Induced by High Glucose in Vitro

摘要: 目的：观察蒙药伊赫汤在体外细胞水平对高糖刺激引起的HMrSV5细胞炎症反应和纤维化的影响。方法：通过ELISA检测伊赫汤处理前后HMrSV5细胞培养体系中炎症相关细胞因子白介素6 (interleukin 6, IL-6)、单核细胞趋化因子1 (monocyte chemoattractant protein 1, MCP-1)、转化生长因子β (transforming growth factor-β, TGF-β)、白介素8 (interleukin 8, IL-8)、白介素17 (interleukin 17, IL-17)和肿瘤坏死因子α (tumor necrosis factor α, TNF-α)含量变化，通过Western blotting检测细胞内纤维化相关蛋白血管内皮生长因子(vascular endothelial growth factor, VEGF)、纤粘蛋白(fibronectin)和IV型胶原(collagen IV)表达量变化。结果：伊赫汤处理组细胞培养基中IL-6、MCP-1、TGF-β、IL-8、IL-17和TNF-α等炎症相关蛋白含量相较于高糖处理组显著降低。HMrSV5细胞经高糖处理后，纤维化相关蛋白fibronectin和collagen IV等在细胞内表达量增加，伊赫汤处理可以显著降低细胞内纤维化相关蛋白fibronectin和collagen IV的表达。结论：伊赫汤可以抑制由高糖处理HMrSV5细胞导致的炎症因子的释放，同时可以显著抑制HMrSV5细胞内纤维化相关蛋白表达，具有抑制腹膜细胞炎症反应和抗纤维化改变作用。

Abstract: Objective: To observe the effect of Mongolian medicine Yihe Decoction on inflammation and fibrosis of HMrSV5 cells stimulated by high glucose in vitro. Methods: The levels of inflammatory cytokines IL-6 (interleukin 6), MCP-1 (monocyte chemoattractant protein 1), TGF-beta (transforming growth factor-β), IL-8 (interleukin 8), IL-17 (interleukin 17) and TNF-alpha (tumor necrosis factor α) in HMrSV5 cell culture system before and after treatment with Yihe Decoction were detected by ELISA, and the expression of intracellular fibrosis-related VEGF (vascular endothelial growth factor), fibronectin, collagen IV were detected by Western blotting. Results: The contents of IL-6, MCP-1, TGF-beta, IL-8, IL-17 and TNF-alpha in cell culture medium of Yihe decoction treatment group were significantly lower than those of high sugar treatment group. The expression of fibronectin and collagen IV in HMrSV5 cells increased after high glucose treatment, and the expression of fibronectin and collagen IV in HMRSV5 cells decreased significantly after treatment with Yihe decoction. Conclusion: Yihe decoction can inhibit the release of inflammatory factors caused by high glucose treatment of HMRSV5 cells, and significantly inhibit the expression of fibrosis-related protein in HMRSV5 cells. It can inhibit the inflammatory reaction of peritoneal cells and anti-fibrosis effect.