基于高通量测序的大豆根际土壤细菌群落的研究

Analysis of Bacterial Communities in Soybean Rhizosphere Based on High Throughput Sequencing

摘要: 大豆根际土壤中微生物群落的变化对大豆植株会产生一定影响，所以研究其变化很有必要。以不同生长阶段的大豆根际土壤为研究对象，利用Illumina HiSeq 2500测序平台对根际土壤中细菌进行测序。经过基因组DNA提取、PCR产物扩增纯化、构建文库以及测序数据分析，物种注释结果表明样品中变形菌门、酸杆菌门、和放线菌门的相对丰度最高。Alpha多样性结果表明成熟期的多样性更加丰富。

Abstract: The changes of microorganisms community soil have important effects on soybean plants, so it is necessary to study the changes. The rhizosphere soil of different growth stages was studied, and the bacteria were sequenced using the Illumina HiSeq 2500 sequencing platform in the rhizosphere soil. After genomic DNA extraction, PCR product amplification and purification, construction of the library and sequencing data analysis, the species annotation of the high throughput sequencing experiment showed that the relative abundance of Proteobacteria, Acidophilus, and Actinomycetes was the highest in the samples. Alpha diversity experiment showed that the diversity is more abundant at maturity.