香梨优斑螟不同虫态内参基因稳定性分析
Expression Stability Analysis of Internal Reference Genes in Insect States of Euzophera pyriella
DOI: 10.12677/HJAS.2019.96063, PDF,  被引量    国家自然科学基金支持
作者: 马光皇*, 符 平, 孙 妍, 康淑媛：塔里木大学，新疆 阿拉尔；杨明禄：塔里木大学，新疆 阿拉尔;农业农村部阿拉尔作物有害生物科学观测站，新疆 阿拉尔
关键词: 香梨优斑螟；内参基因；实时荧光PCR；Euzophera Pyriella； Internal Reference Genes； Real Time Fluorescence PCR
摘要: 香梨优斑螟是新疆危害林果的重要害虫之一，为研究该害虫某些基因的表达情况，选取β-actin、18s rRNA、UBQ、GAPDH和β-tub等5种候选内参基因为研究对象，利用实时荧光定量PCR技术分析候选内参基因相对表达情况，以Delta Ct、GeNorm、BestKeeper、NormFinder和RefFinder软件或方法评价表达稳定性。结果发现：18s rRNA和UBQ基因在香梨优斑螟不同虫态中稳定性较好，只有BestKeeper软件分析UBQ基因稳定性略好于18s rRNA，其次是TUB和β-Actin，GAPDH表达稳定性最差。
Abstract: Euzophera pyriella is one of the important pests in forestry and fruit trees in Xinjiang. In order to study the expression of some genes in this pest, 5 candidate internal reference genes such as β-actin, 18s rRNA, UBQ, GAPDH and β-tub were selected as the research objects. The relative expression of candidate internal reference genes was analyzed by real-time fluorescent quantitative PCR. The expression stability was evaluated by Delta Ct, GeNorm, BestKeeper, NormFinder and RefFinder soft-ware or methods. The results showed that 18s rRNA and UBQ genes had good stability in insect states of Euzophera pyriella, and only BestKeeper software analyzed UBQ gene stability slightly better than 18s rRNA, followed by TUB and β-Actin, GAPDH expression stability was the worst.
文章引用：马光皇, 符平, 孙妍, 康淑媛, 杨明禄. 香梨优斑螟不同虫态内参基因稳定性分析[J]. 农业科学, 2019, 9(6): 427-431. https://doi.org/10.12677/HJAS.2019.96063

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