嗜水气单胞菌处理对斑马鱼补体基因表达水平的影响

Effect of Aeromonas hydrophila Treatment on the Expression of Complement Genes in Zebrafish

摘要: 将斑马鱼饲养在持续充气的自来水中，水温保持在25˚C~27˚C，取15条健康斑马鱼，用108 cells/ml的嗜水气单胞菌溶液浸泡处理24 h，3 h、6 h、12 h和24 h各取3条斑马鱼以实时定量PCR技术检测补体基因的表达水平。A.h处理后，斑马鱼体内C1、C4、C6和MASP的表达水平逐步升高，而C3、Bf和MBL的表达水平则先降低再升高，但它们都在处理6 h或12 h时达到峰值，之后其表达水平逐步降低。这表明鱼类补体系统能在短时间内对外界刺激做出应答，但是由于不同补体成分及补体激活途径在机体免疫中的作用差异，它们对外界感染做出的反应也有所不同。

Abstract: The healthy zebrafish were maintained in well-aerated tap water at 25˚C~27˚C; 15 healthy zebrafish were selected and then immersed in 20 μg/ml Aeromonas hydrophila solution for 24 h; 3 fish were sampled at 3 h, 6 h, 12 h and 24 h; the expression levels of main complement genes were analyzed by real-time fluorescent quantitative PCR. It was shown that the expression of C1r/s, C4, C6 and MASP all up-regulated after A.h treatment and reached the peak at 6 h which was followed by a decline. In contrast, down-regulation of C3, MBL and Bf expression was detected shortly after treatment, and then up-regulation followed and also peaked at 6 h or 12 h. These results indicated that complement system can effectively respond to infection in a short period of time, and different genes respond to the external stimulation in different ways, which might be caused by the different roles of complement components and complement pathways.