枯草芽孢杆菌Bs-W5培养基组分的筛选和培养条件的优化
Screening of Bacillus subtilis Bs-W5 Medium and Optimization of Culture Conditions
DOI: 10.12677/AMB.2019.82012, PDF,    科研立项经费支持
作者: 王 琳：西安医学院医学技术系，陕西 西安；李璐璐, 刘瑞瑞, 李志英：西北大学，西部生物资源与现代生物技术教育部重点实验室，陕西 西安
关键词: 枯草芽孢杆菌；培养基组分；培养条件；Bacillus subtilis； Culture Medium Components； Culture Conditions
摘要: 本文对枯草芽孢杆菌Bs-W5的培养基和培养条件进行了筛选和优化，玉米粉和大豆粉分别作为碳、氮源，添加CaCO3和MnSO4作为促进细菌生长的无机盐。确定摇瓶培养条件为：培养温度设为32℃，摇瓶装液量为75 mL/250mL，接种量为5%，摇床转速设为200 r/min。
Abstract: The culture medium and culture conditions of Bacillus subtilis Bs-W5 were screened and opti-mized in the paper. Corn flour and soybean powder were used as carbon and nitrogen sources respectively, and CaCO3 and MnSO4 were added as inorganic salts to promote bacterial growth. The best shake flask culture conditions are as follows: temperature of 32˚C, liquid volume in flask of 75 mL/250mL, inoculation amount of 5%, rotation speed of 200 r/min.
文章引用：王琳, 李璐璐, 刘瑞瑞, 李志英. 枯草芽孢杆菌Bs-W5培养基组分的筛选和培养条件的优化[J]. 微生物前沿, 2019, 8(2): 93-101. https://doi.org/10.12677/AMB.2019.82012

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