牡蛎富集诺如病毒相关FUT2基因单核苷酸多态性标记开发
Development of SNP Markers for Norovirus Related FUT2 Gene in Oyster
DOI: 10.12677/HJBM.2019.93020, PDF,    国家自然科学基金支持
作者: 闫守泉*, 蒋华生*, 王家丰*：广东医科大学附属医院，干细胞研发与临床转化中心，广东 湛江；宋丽君*：广东医科大学附属医院，生殖医学中心，广东 湛江；周玉兰*：广东医科大学附属医院，临床研究中心，广东 湛江
关键词: 牡蛎；FUT2基因；单核苷酸多态性；诺如病毒； Oyster； FUT2； SNP； Norovirus
摘要: 诺如病毒(NV)可导致急性肠胃炎并具有高度的传染性，一次爆发涉及人数众多，极易引起恐慌。NV致病机理尚不明确，流行病学研究发现牡蛎可特异性富集NV而成为食源感染NV最主要途径，而其生物富集过程机理也不清楚。研究表明人体FUT2多态性会影响NV识别与感染，实验表明牡蛎体内存在类似人体NV识别受体。我们研究发现不同牡蛎个体间NV富集存在显著差异，该差异是否与FUT2多态性相关尚不清楚。本研究拟借助高通量测序等方法筛选牡蛎FUT2多态性位点，然后利用高分辨率熔解曲线以及荧光定量PCR等技术开发、验证FUT2多态性标记。本研究为探索牡蛎富集NV过程机理奠定基础；同时为进一步明确NV感染人体过程以及相关预防和治疗措施提供新思路。
Abstract: Norovirus (NV) may cause acute gastroenteritis with highly contagious, an outbreak of NV infection involves a large number of people which can easily cause panic. However, the pathogenesis of NV infection is unclear by now. Epidemiological studies have found that oysters can specifically enrich NV and thus become the primary approach of food-borne infection of NV, while the mechanism of its biological enrichment process is also not clear. Studies have shown that human FUT2 polymorphism can affect NV recognition and infection. And experiments have discovered the similar NV recognition receptors in oysters. We found significant differences in NV enrichment between different oyster individuals, but it is not clear whether this difference is related to the polymorphism of FUT2 gene. This study intends to screen polymorphic sites of oyster FUT2 gene using high-throughput sequencing, and develop polymorphic markers of oyster FUT2 gene by high-resolution melting curve and fluorescence quantitative PCR. This study laid a foundation for exploring the mechanism of oyster enrichment NV. It also provides new insights into NV infection in humans and contributes to develop prevent and treat methods.
文章引用：闫守泉, 宋丽君, 周玉兰, 蒋华生, 王家丰. 牡蛎富集诺如病毒相关FUT2基因单核苷酸多态性标记开发[J]. 生物医学, 2019, 9(3): 135-142. https://doi.org/10.12677/HJBM.2019.93020

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