银杏叶提取物对心肌细胞H9C2 miR-1，BCL-2和Caspase通路的作用
Effects of Ginkgo Biloba Extract on H9C2 microRNA-1, BCL-2 and Caspase Pathways in Cardiomyocytes
DOI: 10.12677/ACM.2019.97130, PDF,   
作者: 李俊霞, 任明姬：内蒙古医科大学，内蒙古 呼和浩特
关键词: 银杏叶提取物；心肌细胞；bcl-2；Ginkgo Biloba Extract； Myocardial Cells； bcl-2
摘要: 目的：本研究以心肌细胞H9C2为研究对象，对银杏叶提取物对心肌细胞中的miR-1，BCL-2和caspase的表达进行研究，旨在找到银杏叶提取物对心肌细胞的作用机制。方法：通过CCK-8法得到最佳的银杏叶提取物浓度，通过定量PCR检测了miR-1的表达，通过western blot 检测了BCL-2和caspase的表达。结果：当银杏叶提取物的浓度为10 μM的银杏叶提取物在作用36 h时，H9C2细胞的的生长情况最佳。在0，4 h，8 h随着时间的增加miR-1在mRNA水平的表达量逐渐降低(P < 0.05)。在0，4 h，8 h随着时间的增加Bcl-2的表达量逐渐升高(P < 0.05)。而在8 h，12 h在Bcl-2的表达量则没有显著差异(P > 0.05)。在0，4 h，8 h随着时间的增加Bax的表达量逐渐降低(P < 0.05)，而8 h，12 h Bax的表达量没有变化(P > 0.05)。在0，4 h，8 h随着时间的增加caspase 3，caspase 8，caspase 9的表达量逐渐降低(P < 0.05)，而8 h，12 h caspase 3，caspase 8，caspase 9的表达量没有变化(P > 0.05)。结论：初步表明银杏叶提取物对心肌细胞的增殖作用是通过miR-1，Bcl-2和Bax，caspase 3，caspase 8，caspase 9等起作用的。
Abstract: Objective: To investigate the expression of microRNA-1, BCL-2 and caspase in cardiac myocytes induced by Ginkgo biloba extract (GBE) in order to find out the mechanism of action of GBE on cardiac myocytes. Methods: CCK-8 method was used to obtain the optimal concentration of Ginkgo biloba extract. The expression of microRNA-1 was detected by quantitative PCR, and the expression of BCL-2 and caspase was detected by Western blot. Results: The growth of H9C2 cells was the best when the concentration of Ginkgo biloba extract was 10 μM for 36 h. At 0, 4 h, 8 h, the expression of microRNA-1 gradually decreased with time (P < 0.05). At 0, 4 h, 8 h, the expression of Bcl-2 gradually increased with time (P < 0.05). At 8 h, the expression of Bcl-2 at 12 h had no significant difference (P > 0.05). The expression of Bax decreased gradually with the increase of time at 0, 4 and 8 hours (P < 0.05), but did not change at 8 and 12 hours (P > 0.05). The expression of caspase 3, caspase 8 and caspase 9 decreased gradually with the increase of time at 0, 4 and 8 hours (P < 0.05), while the expression of caspase 3, caspase 8 and caspase 9 did not change at 8, 12 hours (P > 0.05). Conclusion: Ginkgo biloba extract has a preliminary effect on the proliferation of cardiac myocytes through microRNA-1, Bcl-2, Bax, caspase-3, caspase-8, caspase-9 and so on.
文章引用：李俊霞, 任明姬. 银杏叶提取物对心肌细胞H9C2 miR-1，BCL-2和Caspase通路的作用[J]. 临床医学进展, 2019, 9(7): 844-851. https://doi.org/10.12677/ACM.2019.97130

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