CARM1通过调控Bax/Bcl-2表达和Caspase-3活性进而调控肝癌生长作用

CARM1 Regulates the Growth of Hepatocellular Carcinoma Cells via Regulation of Bax/Bcl-2 Level and Caspase-3 Activity

摘要: 目的：共激活因子相关精氨酸甲基转移酶1 (Coactivator-associated arginine methyltransferase 1, CARM1)在肝癌细胞中生长作用及机制研究。方法：qRT-PCR、western blot法检测肝癌细胞中CARM1表达；MTT法和台盼蓝实验检测肝癌细胞的生长情况；Caspase-3试剂盒检测Caspase-3活性；小分子干扰RNA干扰CARM1研究其作用和分子机制。结果：小分子干扰RNA明显敲低CARM1水平；干扰CARM1后明显抑制肝癌细胞生长，且下调抑凋亡蛋白Bcl-2和上调促凋亡蛋白Bax表达水平，同时激活Caspase-3活性。结论：CARM1通过调控Bax/Bcl-2表达和Caspase-3活性进而调控肝癌生长作用。

Abstract: Objective: To investigate the effect and mechanism of CARM1 on the growth of HCC. Methods: qRT-PCR and western blot were used to detect the expression of CARM1. MTT assay and Trypan blue assay were used to detect the growth of hepatocellular carcinoma cells; Caspase-3 kit was used to detect the activity of Caspase-3; small interfering RNA interference CARM1 was used to study its role and molecular mechanism on HCC growth. Results: Small interfering RNA significantly knocked down the level of CARM1; interfering with CARM1 significantly, which inhibited the growth of hepatocellular carcinoma cells, down-regulated Bcl-2, up-regulated Bax expression, and activated Caspase-3 activity. Conclusion: CARM1 regulates the growth of hepatocellular carcinoma by regulating the expression of Bax/Bcl-2 and the activity of Caspase-3.