南沙参TLC鉴别和HPLC指纹图谱研究

Study on TLC Identification and HPLC Fingerprint of Adenophora radix

摘要: 目的：对南沙参进行TLC (薄层色谱)鉴别并建立HPLC (高效液相)指纹图谱方法，为其质量控制提供专属性更好的测试方法。方法：TLC采用药典方法；HPLC色谱柱Thermo Acclaim^TM 120 C₁₈ (4.6 × 250 mm, 5 μm)，流动相乙腈-0.5%磷酸水溶液，梯度洗脱，流速为1.0 mL/min，检测波长为203 nm，柱温30℃；采用中药色谱指纹图谱评价系统对不同批次样品进行相似度分析。结果：对9批药材进行了TLC鉴别和建立了9批药材HPLC指纹图谱，确定3个共有色谱峰，方法学验证结果符合指纹图谱相关要求。结论：该方法简单、可靠，可用于南沙参药材的鉴别和质量控制。

Abstract: Objective: To identify Adenophora radix with TLC and to establish HPLC fingerprint of Adenophora radix to provide better specificity test method for the quality control. Methods: Pharmacopoeia TLC method. HPLC Thermo Acclaim^TM 120 C₁₈ (4.6 × 250 mm, 5 μm) was used with acetonitrile-0.5% phosphoric acid water solution as mobile phase in a gradient mode at the flow rate of 1.0 mL/min. The detection wavelength was 203 nm and the temperature of column was set at 30˚C. Similarity evaluation system for chromatographic fingerprint of TCM was applied to analyze different batches of Adenophora radix samples. Results: TLC identification was carried out on 9 batches of medicinal materials, and 9 batches of HPLC fingerprints were established. Three common chromatographic peaks were determined. The results of methodological verification met the related requirements of fingerprints. Conclusion: This method is simple and reliable, and can be used for the identification and quality control of Adenophora radix.