苗药双肾草通过HPA轴调控COPD气道炎症机制的研究

The Research of Miao Medicine Double Kidney Grass Regulation Mechanism of COPD Airway Inflammation through HPA Axis

摘要: 目的：研究双肾草对COPD大鼠下丘脑–垂体–肾上腺轴(HPA轴)的调控，探讨其在COPD大鼠气道炎症治疗中的抗炎作用机理。方法：两次气管内注射脂多糖(LPS)及熏香烟法建立大鼠COPD模型。造模成功后按双肾草煎剂不同剂量灌胃，低剂量组(5 mg/kg)；中剂量组(10 mg/kg)；高剂量组(20 mg/kg)分别灌胃28天。用图像数据分析系统检测平均肺泡内衬间隔(MLI)及肺泡破坏指数(DI)。用Realtime-PCR检测大鼠肺组织中GRmRNA的含量、下丘脑中CRHmRNA及肾上腺中CORTmRNA的含量。结果：双肾草低、中、高剂量组和COPD模型组比较MLI及DI均有显著性差异(P < 0.01)。双肾草低、中、高剂量组和COPD模型组比较CRHmRNA、GCmRNA、CorTmRNA均明显升高(P < 0.05)。GR及CorTmRNA的表达随双肾草剂量的增加而增强，以双肾草高剂量组效果最佳。CRHmRNA的表达各剂量组之间比较无明显统计学差异(P > 0.05)，但实验证明双肾草高剂量组效果优于其他剂量组。结论：双肾草煎剂能够有效的提高CRHmRNA、GRmRNA、CorTmRNA的含量，通过生物反馈效应，提高HPA轴的生物活性，刺激内源性激素的分泌，参与机体免疫，从而抑制COPD炎症反应。

Abstract: Objective: To research the double kidney grass on COPD rats hypothalamus pituitary adrenal axis (HPA axis) control and discuss its anti-inflammatory action mechanism in the treatment of COPD airway inflammation. Methods: Two intratracheal injections of lipopolysaccharide (LPS) and cig-arette smoking were used to establish the COPD model in rats. After the model was successfully established, the rats were given different doses of double kidney grass decoction: low-dose group (5 mg/kg), medium-dose group (10 mg/kg, high-dose group (20 mg/kg) which were given gavage for 28 days. The mean alveolar lining interval (MLI) and alveolar destruction index (DI) were measured using an image data analysis system. The content of GRmRNA in lung tissues, CRHmRNA in hypothalamus and CORTmRNA in adrenal gland was detected by Realtime-PCR. Results: There were significant differences in MLI and DI between low, medium and high dose groups and COPD model group (P < 0.01). CRHmRNA, GCmRNA and CorTmRNA were significantly increased in the low, medium and high dose groups compared to the COPD model group (P < 0.05). The expression of GR and CorTmRNA increased with the increase of the dose of double kidney grass, and the effect was best in the high-dose double kidney grass group. CRHmRNA expression between each dose groups was no significant statistical difference (P > 0.05). But the experiment proved that the double kidney grass effect of high dose group is better than the other groups. Conclusion: Double kidney herb decoction can effectively improve CRHmRNA and CorTmRNA and GCmRNA levels, through the effect of biofeedback, to improve the biological activity of HPA axis, stimulating the secretion of endogenous hormones in the body's immune, thus inhibiting the COPD inflammation.