过敏性紫癜患儿血清白细胞介素35水平变化与临床意义探讨

Changes of Serum Interleukin 35 in Children with Henoch-Schonlein Purpura and Its Clinical Significance

摘要: 目的：观察过敏性紫癜(HSP)患儿急性期血清白细胞介素35 (IL-35)水平变化特点，为探讨HSP的发病机制提供实验依据。方法：选取2017年12月~2018年5月期间住院治疗的HSP患儿49例作为研究对象，62名健康儿童作为正常对照组。应用双抗体夹心法酶联免疫吸附试验(ELISA)检测血清中IL-35的水平。结果：HSP患儿急性期血清IL-35水平为(53.68 ± 9.66) pg/ml；明显低于正常对照组[(65.55 ± 8.64) pg/ml]，差异有显著统计学意义(P < 0.05)。有/无前驱感染的HSP患儿血清IL-35水平分别为(56.61 ± 8.87) pg/ml和(51.98 ± 9.84) pg/ml (P > 0.05)，有/无早期肾损害的HSP患儿血清IL-35水平分别为(52.94 ± 8.80) pg/ml和(54.05 ± 10.17) pg/ml，过敏性紫癜急性期有/无消化道症状的HSP患儿血清IL-35水平分别为(54.10 ± 5.85) pg/ml和(53.50 ± 11.01) pg/ml (P > 0.05)，各组间差异均无显著性(P均 > 0.05)。HSP患儿急性期血清IL-35水平与免疫球蛋白A、G、M、E水平间均无明显相关性(r = −0.226~0.403，P均 > 0.05)。结论：HSP患儿急性期血清IL-35水平降低，负性免疫调节保护作用不足，在儿童HSP的发病机制中可能发挥一定作用。

Abstract: Objective: To observe the characteristics of serum interleukin-35 (IL-35) levels in children with Henoch-Schonlein purpura (HSP) in the acute phase, and to provide experimental basis for exploring the pathogenesis of HSP. Methods: A total of 49 HSP children hospitalized from December 2017 to May 2018 were selected as study subjects, and 62 healthy children as normal control group. The serum IL-35 level was detected by enzyme-linked immunosorbent assay (ELISA) with double antibody sandwich method. Results: The serum IL-35 level of HSP children in the acute phase was (53.68 ± 9.66) pg/ml. It was significantly lower than the control group [(65.55 ± 8.64) pg/ml], and the difference was statistically significant (P < 0.05). Serum IL-35 levels of HSP children with/without prodromal infection were (56.61 ± 8.87) pg/ml and (51.98 ± 9.84) pg/ml (P > 0.05), and those with/without early renal damage were (52.94 ± 8.80) pg/ml and (54.05±10.17) pg/ml, respectively. Serum IL-35 levels of HSP children with/without gastrointestinal symptoms in acute stage of Henoch-Schonlein purpura were (54.10 ± 5.85) pg/ml and (53.50 ± 11.01) pg/ml (P > 0.05), respectively, with no significant difference between each group (all P > 0.05). There was no significant correlation between serum IL-35 level and immunoglobulin A, G, M and E levels (r = −0.226 - 0.403, P = > 0.05). Conclusion: In the acute phase, serum IL-35 level in children with HSP is reduced, and the negative immune regulatory protection is insufficient, which may play a certain role in the pathogenesis of HSP in children.