北虫草多糖粗提物免疫佐剂活性研究

Study on Immune Adjuvant Activity of Crude Extract of Cordyceps Militaris Polysaccharide

摘要: [目的]探讨北虫草多糖作为预防用免疫佐剂的可行性及效果。[方法]以ICR小鼠为模型，经模式抗原OVA免疫后，提取北虫草多糖粗多糖、分别从小鼠体液免疫和细胞免疫两方面进行分析，检测了ICR小鼠48只，随机分成6组，分别为生理盐水组、10 μg抗原组、10 μg抗原混合不同剂量(低、中、高)北虫草粗多糖粗提物以及10 μg抗原加上200 μg铝佐剂作为阳性对照组，小鼠间隔2周皮下免疫2次，二免后2周采血测定血清特异性IgG、IgG亚类、淋巴细胞转化水平检测、细胞因子(IFN-γ, IL-12, IL-4, IL-10) mRNA表达水平。[结果]中剂量组能显著提高血清特异性IgG抗体及亚类水平、特异性T淋巴细胞转化水平、增强小鼠机体Th1/Th2混合型细胞免疫反应(p < 0.05)。[结论]该研究首次证实将北虫草多糖与抗原物质联合使用，能有效提高机体对疫苗的免疫应答反应，将为进一步开发利用北虫草多糖提供科学依据。

Abstract: [Objective] To study the feasibility and effect of Cordyceps militaris polysaccharides as immune adjuvant for prevention. [Method] ICR mice were immunized with OVA model antigen. Cordyceps militaris polysaccharides were extracted and analyzed from both humoral and cellular immunity of mice. Forty-eight ICR mice were randomly divided into six groups: saline group, 10 UG antigen group and 10 UG antigen mixed with different doses (low, medium and high). The crude polysac-charide extract, 10 UG antigen and 200 UG aluminium adjuvant were used as positive control group. The mice were subcutaneously immunized twice at 2 weeks interval. The serum specific IgG, IgG subclasses, lymphocyte transformation level, and the expression of cytokines (IFN-gamma, IL-12, IL-4, IL-10) were detected by blood sampling two weeks after immunization. [Result] The medium dose group could significantly increase the levels of serum specific IgG antibodies and subclasses, specific T lymphocyte transformation and Th1/Th2 mixed cell immune response in mice. [Conclusion] This study proved for the first time that the combined use of Cordyceps militaris polysaccharides and antigen substances can effectively improve the immune response of organisms to vaccines and provide scientific basis for further development and utilization of Cordyceps militaris polysaccharides.