核桃青皮乙醇提取物的抑菌效果测定

The Antibacterial Effect of the Alcoholic Extract from Walnut Green Peel

摘要: 利用核桃青皮组织的乙醇粗提取物对苹果轮纹病菌和桃褐腐病菌的生长抑制效果进行测定。将核桃青皮组织的乙醇提取物稀释10倍，100倍和1000倍，分别测定不同稀释液的抑菌效果，结果表明，10倍稀释提取物的抑菌效果最为显著，10倍和100倍液之间具有极显著差异(P < 0.01)，100倍和1000倍倍液之间在0.01水平和0.05水平上无显著差异(P > 0.05)。取10倍乙醇提取物处理菌液2小时，对菌液中的过氧化氢酶的活性进行鉴定，结果表明核桃青皮提取物对病菌的抗逆性有一定限制，抑制了细胞酶的活性。

Abstract: This research aims to determine the antibacterial effect of the acoholic extract from walnut geen pel. We used Physalospora nasei and Monilinia fructicola to do this study. The acoholic extracts were diluted 10, 100 and 1000 times respectively to determine the antibacterial effect, the results showed that ten times dilution has the significant bacteriostatic effects on Physalospora nasei and Monilinia fructicola, and there were no significant differences between 100 times and 1000 times dilutions at 0.01 level and 0.05 level. Then we continued to use the ten times dilution to deal with the two bacterial solutions to test the catalase activity in the solution, the extract had been proved to have inhibitory effect on catalase activity.